低能N^+注入选育黑曲霉β-葡萄糖苷酶高产菌株及其发酵优化研究  

Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N^+ implantation

作　　者：刁金山[1] 王丽[1] 陈振[1] 刘会[1] 聂光军[1] 郑之明[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,合肥230031

出　　处：《辐射研究与辐射工艺学报》2010年第6期345-351,共7页Journal of Radiation Research and Radiation Processing

基　　金：国家高技术研究发展计划(863计划);(2009AA02Z305)资助

摘　　要：为了获得β-葡萄糖苷酶高产菌株,应用低能N+注入技术对黑曲霉菌株Au进行了诱变选育,同时研究了N+注入对菌株存活率和突变率的影响。通过几轮注入之后,最终筛选得到的高产菌株Au 0847其β-葡萄糖苷酶活力达到13.75 U/mL,比原始菌株Au提高了106.8%,且遗传性能稳定。经过进一步的发酵优化,该菌株最终β-葡萄糖苷酶活力达到30.53 U/mL。Low-energy N＋ implantation was employed to mutate Aspergillus niger Au to enhance productivity of β-glucosidase.Effects of N＋ on strains,survival and mutation rate were studied.After several rounds of implantation,activity of β-glucosidase of the final mutant Au 0847 reached 13.75 U/mL,which is higher by 106.8% than that of original strain Au,and its heritability was stabilized.Activity of β-glucosidase of Au 0847 reached 30.53 U/mL after further fermentation condition optimization.

关 键 词：Β-葡萄糖苷酶 离子注入 黑曲霉 选育 发酵优化 

分 类 号：Q68[生物学—生物物理学] Q939.97