基因治疗脊髓损伤中Ad(腺病毒)-NGF(神经生长因子)的应用研究  

Implication of Ad-NGF in spinal injury's gene therapy

作　　者：杜君好[1] 刘磊[2] 王贵怀[3] 

机构地区：[1]河南省商丘市第一人民医院神经外科二病区,河南商丘477100 [2]北京垂杨柳医院脑外科,北京100022 [3]首都医科大学附属北京天坛医院神经外科三病区,北京100050

出　　处：《中国当代医药》2010年第34期12-13,共2页China Modern Medicine

摘　　要：目的:应用Ad-NGF转染成纤维细胞治疗脊髓半切模型大鼠探讨在基因治疗脊髓损伤中腺病毒作为载体介导的NGF对脊髓损伤的治疗价值。方法:主要材料为Ad-NGF,293细胞,成纤维细胞,大鼠等。成纤维细胞与Ad-NGF悬液混合培养,将细胞注射至脊髓半横切损伤模型大鼠局部。结果:NGF因子在体外实验组可检测到。Ad-NGF转染组单位面积上神经轴突数量比对照组多。结论:NGF因子在体外实验组得到表达,体内修饰组有NGF表达。证实Ad-NGF转染成纤维细胞通过促进脊髓损伤大鼠神经轴突的生长促进损伤神经功能的恢复。Objective：To investigate effects of adenovirus huNGF gene transferd on spinal cord injury in rats.Methods：Main reagent,Ad-NGF,293 cell,fibroblast,rat,etc.Cultivate Ad-NGF with fibroblast,then inject transferred fibroblast into region of spinal cord injury in rats.Results：Got brain tissue from transfected rat,grind,centrifugalization,then got supernatant,after treated by ELISA method,determin extinction value.We found cylindraxile numbers in Ad-NGF transfected group was bigger than other groups.Conclusion：NGF can express in extraorgan group,and in vivo infected group.Ad-NGF transfected fibroblast can promote injured nerve function＇s recovery through promoting cylindraxile＇s grouth.

关 键 词：腺病毒-神经生长因子 成纤维细胞 转染 脊髓损伤 

分 类 号：R-332[医药卫生]