Ad-Rb94联合γ射线照射对食管癌细胞生长的影响  

Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γray on growth of esophageal carcinoma cells

作　　者：陈凤华[1] 李进[1] 谭志军[2] 姜恩海[1] 宋力[1] 王欣茹[3] 王芹[1] 

机构地区：[1]北京协和医学院中国医学科学院放射医学研究所,天津市分子核医学重点实验室,天津300192 [2]天津市第一中心医院外科,300192 [3]北京第二炮兵总医院检验科,100088

出　　处：《国际放射医学核医学杂志》2010年第2期119-121,共3页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家自然科学基金（30770638）

摘　　要：目的 探讨人视网膜母细胞瘤94基因重组腺病毒载体（Ad-Rb94）联合γ射线对人食管癌细胞体外生长的抑制作用.方法 采用随机数字表法将培养细胞分为空白对照组、β-半乳糖苷酶基因重组腺病毒载体（Ad-LacZ）对照组、Ad-Rb94组、γ射线照射组和Ad-Rb94联合γ射线照射组（联合组）,Ad-LacZ和Ad-Rb94于体外转染人食管癌EC109细胞系,转染6 h后进行4 Gy 137Csγ射线照射,用噻唑蓝法检测细胞的生长抑制率.结果 Ad-Rb94组、γ射线照射组和联合组对EC109细胞生长均具有抑制作用,其中,联合组抑制率最高（40.30±4.2）%,明显高于Ad-Rb94组（18.3±0.4）%和γ射线照射组（27.40±2.9）%（x2=7.91,x2=5.82,P＜0.05）;γ射线照射组与Ad-Rb94组比较差异具有统计学意义（x2=5.12,P＜0.05）.结论 Ad-Rb94联合γ射线照射对食管癌细胞的抑瘤作用具有协同效应,能有效地抑制肿瘤细胞的生长.Objective To study the combined effect of exogenous recombinant adenovirus-mediated retinoblastoma gene 94 （Ad-Rb94） combined with γ-ray on the growth of esophageal carcinoma cells. Methods Cell culture were randomly divided into 5 groups： control group, recombinant adenovirus vector containing β-galactosidase gene （ Ad-LacZ ） group, Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γ-ray radiation group. EC109 cells were transfected by Ad-Rb94 and exposed to 4 Gy 137Cs γ-ray irradiation 6 hours after transfection. The inhibition rate of EC 109 cells were detected by MTT assay. Results EC 109 cells transfected with Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γ-ray radiation group were all inhibited. The inhibition rate of Ad-Rb94 combined with γ-ray radiation group reached（40.30%±4.2%）, significantly higher than Ad-Rb94 group （ 18.3%±0.4% ）and γ-ray radiation group （27.40%±2.9%） （x2=7.9 1,x2=5.82, P〈0.05）. The difference of inhibition rate between γ-ray radiation group and Ad-Rb94 group was extremely significant （x2=5.12, P〈0.05 ）. Conclusion The recombinant Ad-Rb94 gene transfection combined with γ-ray shows the synergism for the inhibition of the growth of EC 109 cells.

关 键 词：基因 视网膜母细胞瘤94 食管肿瘤 基因疗法 放射疗法 

分 类 号：R735.1[医药卫生—肿瘤]