不同剂量木糖醇对糖尿病大鼠肾小球Ⅳ型胶原表达的影响  

Effect of xylitol on the collagen-IV expression of glomeruli in diabetic rats

作　　者：沈丽津[1] 尹彦斌[3] 郝久营[2] 王华[4] 孙丽荣[1] 

机构地区：[1]天津医科大学代谢病医院糖尿病痛风科,300070 [2]天津医科大学代谢病医院检验科,300070 [3]天津第三中心医院急诊科,300170 [4]天津市妇女儿童保健中心,300074

出　　处：《国际内分泌代谢杂志》2010年第6期371-373,共3页International Journal of Endocrinology and Metabolism

基　　金：基金项目：天津市自然科学基金资助项目（07JCYBJC11400）

摘　　要：目的探讨不同剂量木糖醇对糖尿病大鼠肾小球Ⅳ型胶原表达的影响。方法将40只大鼠随机分为5组（每组8只）：正常对照组（NC组），糖尿病对照组（DC组），糖尿病5％木糖醇组（低剂量组），糖尿病10％木糖醇组（中剂量组），糖尿病20％木糖醇组（高剂量组）。第8周末处死大鼠，用反相高效液相法（RP—HPLC）测定每组大鼠血清尿酸水平，取肾脏测定肾小球Ⅳ型胶原（Col-Ⅳ）免疫组化表达情况。结果实验8周末糖尿病各组血清尿酸水平均较NC组升高（P〈0．05）；低、中剂量组血清尿酸水平高于DC组（P〈0．05）；高剂量组血清尿酸水平较DC组下降（P〈0．05）。肾小球Col—Ⅳ表达比较：实验8周末糖尿病各组与NC组相比呈高表达（P〈0．05）；低、中剂量组与DC组相比呈高表达（P〈0．05），高剂量组则呈低表达（P〈0．05）。结论低、中剂量木糖醇致血尿酸水平升高，肾小球Col—Ⅳ的积聚增加，可加重糖尿病肾病。高剂量木糖醇可使血尿酸水平降低．肾小球Col-Ⅳ的积聚减少、使糖尿病肾病有所缓解。Objective To observe the effect of xylitol on the collagen-Ⅳ expression of glomeruli in diabetic rats. Methods Fourty Wistar rats were randomly divided into five groups（8 rats in each grarp） ： normal control group（ group NC）,diabetes control group （group DC） , 5% xylitol-treated group （group lowdose）, 10% xylitol-treated group （group medium-dose） and 20 % xylitol-treated group （group high-dose）. At the end of 8 weeks, the level of serum uric acid in each group was tested using reversed-phase high performance liquid chromatography. The rats were sacrificed, and the collagen-Ⅳ expression of glomeruli were determined by immunohistorieal chemistry. Results At the end of 8 weeks,the concentration of serum uric acid was increased in the diabetes groups compared with group NC. Group low-dose and group medium- dose had higher concentration of serum uric acid compared with group DC （ P 〈 0.05 ）. Whereas, group highdose had lower concentration of serum uric acid compared with group DC （ P 〈 0.05 ）. The expression of Col- Ⅳ in glomeruli was up-regulated in all diabetes groups at the end of 8 weeks （ P 〈 0.05 ）. Compared with group DC, the expression of Col-Ⅳ was significantly increased in group low-dose and group medium-dose, and decreased in group high-dose （ P 〈 0.05 ）. Conclusions Low and medium dose of dietary xylitol supplementation could raise the level of serum uric acid and deposition of Col-Ⅳin glomeruli. Diabetic nephropathy would be worsen. High dose of xylitol can decrease the level of serum uric acid, as well as the expression of Col-Ⅳ in glomeruli. The injuries to the kidney could be protected.

关 键 词：木糖醇 糖尿病肾病 尿酸 Ⅳ型胶原 

分 类 号：R692[医药卫生—泌尿科学] R587[医药卫生—外科学]