绣球菌驯化栽培  

Artificial Cultivation of Sparassis crispa

作　　者：贾培培[1] 卢伟东[1] 郭立忠[1] 姜辉[1] 王胜蕊[1] 

机构地区：[1]青岛农业大学应用真菌省级重点实验室,山东青岛266109

出　　处：《食用菌学报》2010年第3期33-36,共4页Acta Edulis Fungi

基　　金：山东省良种产业化项目"山东设施名菜及食用菌新品种选育"(编号:NYLZ2009-06)的部分研究内容

摘　　要：从野生的绣球菌（Sparassis crispa）子实体中分离纯化绣球菌菌株,通过平板试验、液体发酵试验筛选绣球菌菌丝生长的最佳固体和液体培养基,并用预处理过的松木屑等为材料栽培绣球菌,结果表明：最佳培养基分别为M6（啤酒酵母1.0%、蜂蜜粉0.3%、蛋白胨0.1%、大豆粉0.1%、KH2PO40.1%、琼脂2%p、H 4.5）、L4（M6去掉琼脂）;菌丝在23-25℃条件下培养,约2个月显蕾,菇蕾在12-15℃培养,约4个月得到成熟的子实体,生物转化率约57%。A strain of Sparassis crispa was isolated and purified from a fruit body collected in the wild.Fungal mycelium grew optimally on a solid medium（M6） consisting of（w/w） brewers yeast 1.0%、honey powder 0.3%、peptone 0.1%、soybean meal 0.1%、KH2PO4 0.1%、agar 2%、pH 4.5,and on L4 liquid medium（M6 medium without added agar）.When cultivated on pretreated sawdust,the strain grew optimally at 23-25 ℃ and formed buds.After cultivation at 12-15 ℃ for a further four months,mature fruit bodies were formed and biotransformation rates up to 57% were achieved.

关 键 词：绣球菌 固体培养基 液体培养基 培养料 人工栽培 

分 类 号：S646[农业科学—蔬菜学]