单增李斯特菌四环素耐药基因tetM膜接合转移的研究  

Study on filtermating transfer of tetracycline resistant gene tetM in Listeria monocytogenes

作　　者：邵美丽[1,2] 刘思国[3] 尹录[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院博士后工作站,黑龙江哈尔滨150086 [3]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国兽医科学》2010年第6期589-592,共4页Chinese Veterinary Science

基　　金：东北农业大学博士启动基金(2006-12);黑龙江省科技厅青年基金(QC08C100);农业部公益行业科研专项(200903012)

摘　　要：为探讨猪链球菌能否成为单增李斯特菌四环素耐药基因tetM的水平传播宿主,以携带该基因的单增李斯特菌四环素耐药菌株为供体菌株,猪链球菌的红霉素耐药菌株为受体菌株进行滤膜接合试验。同时对供体菌株进行了转移相关基因int-Tn的PCR检测。结果显示,获得19个接合子,接合转移率为4×10-7。接合子均对四环素耐药,且接合子中均PCR扩增到tetM基因,该基因克隆测序结果与供体菌中tetM基因序列完全一致。同时,从供体菌株中扩增到整合酶编码基因int-Tn。这说明猪链球菌可以成为单增李斯特菌四环素耐药基因tetM的水平传播宿主,并且该基因的水平转移与整合子有密切关系。To explore the possibility of Streptococcus suis as the horizontal transmission host of tetracycline resistant gene tetM in Listeria monocytogenes,the filtermating experiment was performed in which tetracycline-resistant L.monocytogenes strain carrying tetM gene was used as the donor strain and erythromycin-resistant S.suis strain as recipient strain.The int-Tn gene related to transfer of tetM gene was detected from the donor strain by PCR.19 transconjugants resistant to tetracycline were obtained at a frequency of 4×10-7.The tetM gene was amplified from transconjugants,which shared 100% homology in nucleotides with tetM gene from donor strain.The int-Tn gene that encoded integrase was amplified from the donor strain.The results demonstrated that S.suis could become the horizontal transmission host of tetracycline resistant gene tetM in L.monocytogene,and horizontal transfer of tetM gene was closely related to integron.

关 键 词：单增李斯特菌 猪链球菌 四环素 耐药 基因水平转移 

分 类 号：S852.612[农业科学—基础兽医学]