胶质瘤组织中Nanog基因的表达及其意义  

Expression and Clinical Significance of Nanog in Gliomas

作　　者：李冬雪[1] 牛朝诗[1,2,3] 刘于海[1] 高歌[1] 费小瑞[1] 李明武[1] 董永飞[1] 

机构地区：[1]安徽医科大学附属省立医院神经外科 [2]安徽医科大学附属省立医院神经分子生物学与神经再生修复研究室 [3]安徽省脑立体定向神经外科研究所,安徽合肥230001

出　　处：《中国神经肿瘤杂志》2010年第1期9-13,共5页Chinese Journal of Neuro-Oncology

基　　金：国家自然科学基金资助项目(No.30672166)

摘　　要：背景与目的:Nanog作为全能性或多能性干细胞标志物,在胶质瘤中的研究甚少。本研究探讨Nanog基因在不同病理级别胶质瘤组织中的表达及其意义。方法:采用免疫组织化学染色法和逆转录-聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)方法从蛋白和mRNA水平检测50例不同病理级别胶质瘤组织和7例正常脑组织标本中Nanog的表达。结果:Nanog蛋白在胶质瘤组织WHOⅡ级、Ⅲ级、IV级的阳性率分别为(18.3±9.6)%、(37.9±19.0)%、(53.4±19.8)%,差异有统计学意义(F=14.918,P=0.000);Nanog mRNA的相对含量在胶质瘤组织WHOⅡ级、Ⅲ级、IV级中分别为0.1824±0.0310、0.3730±0.0961、0.4594±0.0453,差异有统计学意义(F=65.901,P=0.000),Nanog蛋白和mRNA表达强度随着病理级别增高而升高,而在正常脑组织中均未见表达。结论:Nanog在胶质瘤组织中高表达,其表达强度与病理级别密切相关,为进一步研究其与肿瘤干细胞关系及其在胶质瘤的生物学行为中所发挥的作用奠定了基础。BACKGROUND OBJECTIVE：Nanog,as a marker of totipotent or pluripotent stem cells,has been rarely investigated in gliomas.In this article,we explored the expression and clinical significance of Nanog in gliomas with different pathological grade.METHOD：The expression of Nanog mRNA was examined by reverse transcription polymerase chain reaction（RT-PCR） and Nanog protein was detected by immunohistochemistry in the pathological specimens from 50 cases of glioma tissues and 7 cases of normal brain tissues respectively.RESULT：The expression rate of immunopositive cells was（18.3±9.6）% in WHO GradeⅡ,（37.9±19.0）% in WHO Grade Ⅲ,（53.4±19.8）% in WHO Grade Ⅳ,respectively（F=14.918,P=0.000）.The relative mRNA level of Nanog was 0.1824± 0.0310 in WHO GradeⅡ,0.3730±0.0961 in WHO Grade Ⅲ,0.4594±0.0453 in WHO Grade Ⅳ respectively（F= 65.901,P=0.000）.The relative level of Nanog expression is positively correlated with the pathological grade.The Nanog protein and mRNA were not detected in normal brain tissues.CONCLUSION：Nanog is overexpressed in gliomas.The relative level of Nanog expression is correlated with the pathological grade.The overexpression of Nanog suggests that it might contribute to the existence of brain tumor stem cells,which provides a clue to the further explore its role in biological behavior of gliomas.

关 键 词：胶质瘤 NANOG 肿瘤干细胞 表达 

分 类 号：R739.41[医药卫生—肿瘤]