重组人生长激素联合氟尿嘧啶体外干预人结肠癌细胞株增殖  

The effects of recombinant human growth hormone and 5-FU on the growth of colon cancer cells in vitro

作　　者：高潺潺[1] 侍方方[1] 王琳[2] 陆颖芝[1] 李苏宜[1] 

机构地区：[1]东南大学附属中大医院肿瘤科,江苏南京210009 [2]海南省人民医院肿瘤科,海南海口570000

出　　处：《肠外与肠内营养》2010年第6期355-358,共4页Parenteral & Enteral Nutrition

基　　金：海南省自然科学基金项目(806112)

摘　　要：目的:在体外环境下观察基因重组人生长激素(rhGH)及联合氟尿嘧啶(5-FU)干预不同细胞膜生长激素受体(GHR)表达水平对人结肠癌细胞的增殖情况。方法:采用免疫细胞化学法检测人结肠癌细胞株HCT-8和LOVO膜表面的GHR表达水平。每株细胞分为对照组、rhGH组、5-FU组和rhGH+5-FU组,用四甲基偶氮唑蓝(MTT)、克隆形成实验和免疫细胞化学等方法,测定各组各株肿瘤细胞的抑制率、增殖细胞核抗原(PCNA)表达变化。结果:HCT-8高表达GHR(阳性表达细胞数≥50%),LOVO未表达GHR。rhGH显著提高HCT-8细胞增殖和克隆形成率,PCNA表达增加,且联合化疗后,较LOVO组肿瘤抑制率减少(P<0.05)。结论:在体外环境下,rhGH促GHR阳性表达的结肠癌细胞增殖,且对5-FU化疗有抵抗,对于不表达GHR的结肠癌细胞无上述作用。Objective： To investigate the effect of recombinant human growth hormone（rhGH） and 5-FU on the growth of colon cancer cells with dfferent expression of growth factor receptor（GHR） in vitro.Methods： The expression level of membrane GHR of colon cancer cell line HCT-8 and LOVO were measured by immune cellular chemistry.The two cell lines were divided into 4 different groups according to the different treatment as indicated：control group,rhGH group,rhGH＋5-FU group and 5-FU group.Cell growth/inhibition rates were determined by MTT,colony formation and PCNA were measured by immune cellular chemistry.Results： HCT-8 showed high level GHR（≥50%）,LOVO showed no GHR expression,and rhGH increased the growth ability of HCT-8 cells.Compared with LOVO,HCT-8 cells showed less growth inhibition under the stimulation of rhGH and more PCNA expression.Conclusion： rhGH can stimulate GHR positive colon cancer cell growth,protect the cell growth inhibition from chemoraphy,and this effect was not observed in GHR negative colon cancer cells.

关 键 词：重组人生长激素 生长激素受体 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]