藏药十五味萝蒂明目丸对紫外线诱导的白内障大鼠晶状体上皮细胞的保护作用  

Protective effect of Shi wu wei luo di ming mu wan of Tibetan medicine on lens epithelial cells of ultraviolet-induced cataract in rats

作　　者：李伟华[1] 亢泽峰[2] 

机构地区：[1]新乡医学院第三附属医院,河南省新乡市453003 [2]青海省卫生厅,青海省西宁市710001

出　　处：《眼科新进展》2010年第12期1105-1107,1110,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(No:30772889)~~

摘　　要：目的探讨藏药十五味萝蒂明目丸(shi wu wei luo diming mu wan of Tibetan medicine)对紫外线诱导的白内障大鼠晶状体上皮细胞(lens epithelial cells,LECs)的保护作用。方法将90只SD大鼠随机分为正常对照组、模型组、阳性药物组(吡诺克辛滴眼液)、藏药高剂量组、藏药低剂量组和预防组,以紫外线诱导大鼠白内障模型后予以治疗,预防组自造模第一天同时给药。利用逆转录聚合酶链反应检测给药后50d、70d、90d LECs内抗氧化蛋白基因表达的水平,并在透射电镜下观察LECs的超微机构,进一步证明十五味萝蒂明目丸对紫外线诱导的白内障LECs是否具有保护作用。结果应用不同剂量的十五味萝蒂明目丸对混浊的晶状体进行干预,随着时间的延长抗氧化蛋白基因表达水平升高,透射电镜下细胞损伤的程度减轻,其中治疗组[藏药高剂量组50d时抗氧化蛋白基因水平为(29.00±0.05)]效果优于阳性药物组,以预防组效果最为明显。结论藏药十五味萝蒂明目丸对紫外线诱导的白内障LECs具有较好的保护作用,并起到预防及治疗效果。Objective To investigate the protective effect of Shi wu wei luo di ming mu wan of Tibetan medicine on protective effects of lens epithelial cells（LECs）of ultraviolet-induced cataract in rats.Methods A total of 90 SD rats were randomly divided into normal control group,model group,positive drug group（pirenoxine eye drops）,Tibetan medicine at high dose group,Tibetan medicine at low dose group and preventive group.After ultraviolet-induced cataract models in rats,treatment was taken.Rats in preventive group were taken drug treatment at the first day after modelling.Levels of antioxygen protein gene expression in LECs were detected at 50 days,70 days and 90 days after drug treatment by reverse transcription polymerase chain reaction.Microstructures of LECs were observed under transmission electron microscope,and to further determine the protective effect of Shi wu wei luo di ming mu wan of Tibetan medicine on LECs of ultraviolet-induced cataract.Results After using different doses of Shi wu wei luo di ming mu wan of Tibetan medicine to treat cloudy lens,level of antioxygen protein gene expression increased with the time,and the level of cell injury decreased under transmission electron microscope.The effect of treatment group（The level of antioxygen protein gene expression was 29.00±0.05 at 50 days in Tibetan medicine at high dose group）was better than that of positive drug group,and the effect of preventive group was the most obvious.Conclusions Shi wu wei luo di ming mu wan of Tibetan medicine has protective effect on LECs of ultraviolet-induced cataract.It is with preventive and curative effect.

关 键 词：藏药十五味萝蒂明目丸 紫外线 白内障 晶状体上皮细胞 保护作用 

分 类 号：R776[医药卫生—眼科]