自噬参与氧化性低密度脂蛋白诱导的血管平滑肌细胞钙化  

Autophagy is Involved in Progression of Vascular Smooth Muscle Cell Calcification Induced by Ox-LDL

作　　者：陆立鹤[1] 颜建云[2] 于汇民[3,4] 刘筱霭[2] 

机构地区：[1]中山大学中山医学院病理生理教研室,广东广州510080 [2]广州医学院生理教研室,广东广州510185 [3]广东省人民医院心内科 [4]广东省医学科学院广东省心血管病研究所,广东广州510080

出　　处：《中山大学学报（医学科学版）》2010年第6期772-775,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81000124);广东省医学科研基金(A2010037)

摘　　要：【目的】观察细胞自噬是否参与了氧化型低密度脂蛋白(Ox-LDL)诱导的血管平滑肌细胞钙化。【方法】采用体外血管平滑肌细胞钙化模型,实验分为3组:control组:用nativeLDL处理细胞;Ox-LDL组:用Ox-LDL处理细胞;3MA组:用Ox-LDL和3MA处理细胞;细胞培养液为DMEM+10mMBGP。分别以茜素红检测血管钙化,Q-PCR和Westernblotting测定cbfa1和Beclin1的mRNA和蛋白表达。【结果】Ox-LDL加速血管平滑肌细胞钙化,上调cbfa1和Beclin1的表达;细胞自噬特异性抑制剂3MA减弱细胞钙化,下调cbfa1和Beclin1的表达。【结论】Ox-LDL诱导的血管平滑肌细胞钙化与细胞自噬有关。【Objective】 To determine whether autophagy is involved in the process of vascular smooth muscle cell calcification induced by Ox-LDL. 【Methods】 The in vitro model of vascular calcification was used in this study. Vascular smooth muscle cells were randomly divided into controls,an Ox-LDL-treated group,and a 3MA plus Ox-LDL-treated group. Cells were grown in DMEM supplemented with 10 mM BGP. Calcification was assessed by alizarin red staining. The mRNA and protein expression of cbfa1 and Beclin1 were analyzed by Q-PCR and Western blotting respectively. 【Results】 Ox-LDL promotes vascular smooth muscle cell calcification,up-regulates the mRNA and protein expression of cbfa1 and Beclin1. The specific autophagy inhibitor attenuates vascular calcification,and inhibits the up-regulation of cbfa1 and Beclin1 expression induced by Ox-LDL. 【Conclusion】 The pathogenesis of vascular smooth muscle cell calcification induced by Ox-LDL involves autophagy.

关 键 词：氧化型低密度脂蛋白 自噬 血管平滑肌细胞 钙化 

分 类 号：R364[医药卫生—病理学]