北京市2009年手足口病的病原构成及柯萨奇A组16型病毒基因特征分析  

Etiology of Hand-Foot-and-Mouth Disease and Genetic Characteristics of Coxsackievirus A16 in Beijing,2009

作　　者：曲梅[1,2] 李洁[1,2] 贾蕾[1,2] 檀晓娟[3] 高志勇[1,2] 严寒秋[1,2] 郭婧[1,2] 李锡太[1,2] 黎新宇[1,2] 王全意[1,2] 许文波[3] 黄芳[1,2] 

机构地区：[1]北京市疾病预防控制中心传染病与地方病控制所,北京100013 [2]首都医科大学,北京100069 [3]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《病毒学报》2010年第6期432-436,共5页Chinese Journal of Virology

基　　金：国家973项目课题"EV71等病毒变异与毒力关系的研究"(2011CB504902);北京市卫生局青年科研资助项目"北京地区手足口病原谱和病原体变异研究"(2009);北京市科技新星计划(2008B28)

摘　　要：阐明北京市2009年手足口病(Hand-Foot-and-Mouth Disease,HFMD)的主要病原构成并对柯萨奇病毒组16型(Coxsackievirus A16,CoxA16)进行基因特征分析。用逆转录聚合酶链反应(Reverse transcriptase polymerase chain reaction,RT-PCR)方法,对2009年从北京市儿童医院、北京佑安医院、北京地坛医院就诊的975例病例中采集的1044份临床标本进行肠道病毒核酸检测;采用人横纹肌肉瘤(Human rhabdom yosarcoma,RD)细胞对肠道病毒核酸检测阳性的200份咽拭子标本进行病毒分离培养鉴定,并对9株CoxA16分离株进行VP1区编码区序列测定和分析。结果表明2009年北京市HFMD的主要病原为CoxA16(49.4%)和EV71(36.4%),其中CoxA16为优势血清型;此外,还有其它型别的肠道病毒(CoxA4、CoxA10和CoxA9等)共流行。基因分型表明2009年北京流行的CoxA16属于B1a和B1b基因型。This study aimed to reveal the pathogen spectrum of hand-foot-and-mouth disease（HFMD） and genetic characteristics of Coxsackievirus A16（CoxA16） isolates in Beijing in 2009.From 1044 clinical specimens collected from 975 HFMD cases at Beijing Pediatrics Hospital,Beijing You＇an Hospital and Beijing Ditan Hospital in 2009,viral nucleic acids of enterovirus were detected by reverse transcriptase polymerase chain reaction（RT-PCR）.Enterovirus isolations were conducted with rhabdomyosarcoma（RD） cell line on 200 throat swabs having positive RT-PCR results.Sequencing and analyses of VP1 encoding gene were performed on 9 CoxA16 isolates in this study.The results showed that CoxA16（49.4%） and EV71（36.4%）were the major pathogens for the epidemics of HFMD in 2009 in Beijing,and CoxA16 was the predominant serotype,while there were also other enterovirus co-circulating,such as CoxA4,CoxA10,and CoxA9;the CoxA16 strains prevalent in Beijing in 2009 belonged to subgenotype B1a and B1b.

关 键 词：手足口病 柯萨奇病毒A组16型(CoxA16) 基因型 

分 类 号：R373.2[医药卫生—病原生物学]