CCL18趋化性细胞因子对A375黑素瘤细胞株增殖和侵袭的影响  

Effects of chemokine CCL18 on the proliferation and invasion of a human melanoma cell line A375

作　　者：辛崇美[1] 李光[1] 孙建方[1] 姜祎群[1] 陈浩[1] 曾学思[1] 徐秀莲[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2010年第12期833-836,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30700727）;国家教委高校博士点基金（20070023113）

摘　　要：目的 探讨CCL18对A375黑素瘤细胞株增殖、侵袭及血管增殖的影响.方法 提取成人外周血单核细胞,IL-4诱导48 h后,与A375黑素瘤细胞株共培养,通过MTT法检测CCL18对A375增殖的影响;通过趋化试验及重组基底膜侵袭实验,测定CCL18对肿瘤细胞的趋化能力及侵袭作用的影响.通过将A375黑素瘤细胞株接种于鸡胚尿囊膜,测定不同情况下CCL18对肿瘤血管生成的影响.结果 IL-4诱导单核细胞组、非诱导单核细胞组呈现促进A375细胞增殖的作用,而CCL18与对照组比较差异无统计学意义.IL-4诱导单核细胞组及CCL18重组细胞因子组与对照组比较均对肿瘤细胞存在趋化作用,并促进肿瘤细胞的侵袭（P＜0.05）;IL-4诱导组及CCL18组在鸡胚尿囊膜试验中可促进肿瘤血管的生成（P＜0.05）.结论 CCL18具有促进A375黑素瘤细胞株侵袭及血管增殖的作用.Objective To investigate the effects of chemokine CCL18 on the proliferation and invasion of a human melanoma cell line A375. Methods Human peripheral blood monocytes were isolated from healthy volunteers, cultured in vitro and divided into two groups to be induced by IL-4 for 48 hours or remain untreated. A375 cells were classified into 3 groups to be cultured with IL-4-induced monocytes, untreated monocytes or CCL18 of 200 g/L for various durations. A375 cells receiving no treatment served as the control.MTT assay was performed to detect the proliferation of A375 cells, chemotaxis test and Matrigel-transwell assay to evaluate the chemotaxis and invasion ability of A375 cells, and chicken chorioalllantoic memebrane （CAM）was used to detect the effect of CCL18 on tumor angiogenesis. Results The proliferation of A375 cells was statistically accelerated by IL-4-induced monocytes and untreated monocytes, but unaffected by CCL18.Matrigel-transwell assay revealed that IL-4-induced monocytes and CCL18 promoted the chemotaxis and invasion ability of A375 cells （all P〈0.05）. Tumor angiogenesis was also increased by IL-4-induced monocytes and CCL18 （both P 〈 0.05）. Conclusion The chemokine CCL18 can promote the invasion ability of A375 cells and tumor angiogenesis.

关 键 词：黑色素瘤 实验性 趋化性细胞因子CCL18 细胞增殖 

分 类 号：R739.5[医药卫生—肿瘤]