胰岛素样生长因子结合蛋白-4在围植入期小鼠子宫内膜的表达及意义  

Expression of insulin-like growth factor binding protein-4 in the peri-implanation mouse endometrium

作　　者：叶希平[1] 陈晓蓉[1] 张荣宜[1] 高晓妹[2] 

机构地区：[1]安徽医科大学组织胚胎学教研室,合肥230032 [2]安徽医科大学第一临床学院,合肥230032

出　　处：《安徽医科大学学报》2010年第6期743-747,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金重点项目(编号:KJ2007A034)

摘　　要：目的检测胰岛素样生长因子结合蛋白-4(IGFBP-4)在围植入期小鼠子宫内膜的表达分布及其生物学作用。方法取未孕动情期、真孕及假孕3～6d小鼠子宫做切片,采用免疫组化SP法检测IGFBP-4在小鼠子宫内膜的表达情况。结果 IGFBP-4在小鼠子宫内膜主要分布于腺上皮细胞、腔上皮细胞、基质细胞及蜕膜细胞。IGFBP-4在真孕组小鼠子宫内膜于孕3d出现阳性表达;孕4～5d中等强度表达;孕6d表达最强,主要分布于整个蜕膜区细胞。未孕动情期小鼠子宫内膜IGFBP-4呈弱表达。假孕组小鼠子宫内膜IGFBP-4只在孕5～6d有微弱表达。真孕组小鼠子宫内膜IGFBP-4表达明显强于同期假孕组。结论 IGFBP-4在围植入期小鼠子宫内膜持续表达,提示IGFBP-4可能在小鼠胚泡植入过程发挥重要生物学作用。Objective To observe the expression and distribution of insulin-like growth factor binding protein-4 （IGFBP-4） and its biological effects in the peri-implantation mouse endometrium.Methods Uterus of mice in the nonpregnant （estrus stage） ,pregnancy and pseudopregnant 3 to 6 days were obtained.Immunohistochemical SP staining method was used for detecting the expression of IGFBP-4 in the endometrium during mice embryo impantation.Results The expression of IGFBP-4 was distributed in the glandular epithelium,luminal epithelium,stromal cells and decidual cells.In the pregnant group,the expression of IGFBP-4 in the uterine endometrium on the 3rd day began to appear,increased in 4th day and 5th day,peaked at the 6th day,mainly distributed throughtout decidua cells.In the pseudopregnant group,weak expression of IGFBP-4 was only observed on the 5 th to 6th days of pregnancy.The expression of IGFBP-4 was weakly in the endometrium of nonpregnant mice.The expression of IGFBP-4 mice in endometrium of the pregnant group was significantly higher than that in the homeochronous pseudopregnancy group.Conclusion IGFBP-4 expresses continuously in mouse endometrium during day 3 ～ 6 of pregnancy,It suggests that IGFBP-4 might play an important biological role in the process of the mouse embryo implantation.

关 键 词：胰岛素样生长因子结合蛋白4 胚胎植入 子宫内膜 小鼠 

分 类 号：R341[医药卫生—基础医学] R321.3