重组腺病毒介导的sTLR2对LPS致炎小鼠抗炎效应的实验研究  

Experimental study on anti-inflammatory effect of adenovirus-mediated sTLR2 in LPS-induced murine inflammation

作　　者：孙守勋[1] 李强[2] 石妍[3] 刘静[2] 蒲晓允[2] 

机构地区：[1]解放军第201医院检验科,辽宁辽阳111000 [2]第三军医大学附属新桥医院检验科,重庆400037 [3]解放军第201医院麻醉科,辽宁辽阳111000

出　　处：《细胞与分子免疫学杂志》2010年第12期1186-1188,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：全军"十一五"面上项目(2006B060);"创伤;烧伤与复合伤国家重点实验室"开放基金资助项目(2006B-2)

摘　　要：目的:观察携带Toll样受体2(TLR2)胞外区基因的重组腺病毒介导的sTLR2在小鼠体内的抗炎作用,为急性炎症反应的防治提供新的思路和治疗策略。方法:建立亚致死剂量脂多糖(LPS)所致炎症小鼠模型。将BALB/c小鼠随机分为正常对照组、炎症对照组及重组腺病毒(Ad)TLR2实验组。AdTLR2于致炎前后,分别作用于小鼠,观察其对小鼠生存时间的影响,并应用ELISA对血清TNF-α、IL-1β、IL-6、IL-10和IL-13的含量进行检测,探讨AdTLR2对小鼠血清细胞因子水平变化的影响。结果:重组AdTLR2作用于炎症小鼠模型后,实验组小鼠的生存时间与炎症对照组比较相对延长,并且实验组中血清TNF-α、IL-1β、IL-6、IL-10和IL-13的水平均较炎症对照组显著降低(P<0.05)。结论:重组AdTLR2能够使LPS所致炎症小鼠模型的生存时间相对延长,并能够降低炎症小鼠血清中TNF-α、IL-1β、IL-6、IL-10和IL-13的含量,提示AdTLR2在活体内可以分泌sTLR2蛋白发挥免疫调节作用并能降低血清中炎性细胞因子的水平,具有一定的抗炎作用。AIM： To observe the anti-inflammatory effect of adenovirus-mediated sTLR2 in LPS-induced murine inflammation,and to provide a new thinking and tactics of prevention and treatment for acute inflammatory reaction.METHODS： Murine inflammation model induced by LPS（lethal dose） was established.BALB/c mice were divided into normal control group,inflammation model group and AdTLR2 treatment group.The recombinant adenovirus（Ad）TLR2 acted on test mice respectively before and after inflammation production,the impact of AdTLR2 on murine survival time was observed.Serum samples of different experiment groups were collected,alteration of cytokine TNF-α,IL-1β,IL-6,IL-10 and IL-13 were tested by ELISA,the influence of AdTLR2 on serum level of cytokines were evaluated.RESULTS： A prolonged survival time by AdTLR2 was observed on lethal dose of LPS animal model;serum level of TNF-α,IL-1β,IL-6,IL-10 and IL-13 were reduced by AdTLR2 on LPS induced murine model,indicating a certain immuno-regulation and anti-inflammatory effect of recombinant AdTLR2.CONCLUSION： Recombinant AdTLR2 resulted a prolong survival time of LPS induced inflammation animal model and reduction of serum level of TNF-α,IL-1β,IL-6,IL-10 and IL-13,which achieved a certain immuno-regulation and anti-inflammatory effect.

关 键 词：TOLL样受体2 LPS 腺病毒 抗炎 细胞因子 

分 类 号：R364.5[医药卫生—病理学]