亚砷酸钠对人脐静脉内皮细胞迁移、增殖的影响及其机制  

Effect and mechanism of sodium arsenite in the migration and proliferation of HUVECs

作　　者：吕慧[1] 史艳芬[1] 仲苓芝[1] 李玉林[1] 李荣贵[1] 

机构地区：[1]吉林大学白求恩医学院病理学系,长春市130021

出　　处：《江苏医药》2010年第22期2663-2665,I0001,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(3087293);吉林大学留学回国科研启动基金[科技字(419)号]

摘　　要：目的探讨亚砷酸钠对体外培养的人脐静脉内皮细胞(HUVECs)迁移和增殖的影响。方法通过细胞损伤修复实验和细胞增殖实验分别观察亚砷酸钠对细胞迁移、增殖能力的影响。RT-PCR分析其mRNA表达。结果亚砷酸钠抑制HUVECs迁移和增殖能力,两者均呈典型的剂量-效应关系。并发现亚砷酸钠下调HUVECs的黏着斑激酶(FAK)和原癌基因蛋白(c-Myc)的mRNA水平。结论亚砷酸钠通过下调FAK和c-Myc基因表达抑制细胞迁移和增殖能力,提示其在损伤血管内皮细胞修复机能中起重要作用。Objective To explore the effect and mechanism of sodium arsenite in the migration and proliferation of human umbilical vein endothelial cells（HUVECs）.Methods Woud healing and proliferation tests were applied to evaluate injury repair fuctions of the cells and RT-PCR was used to analyze the expression of their mRNA.Results The migration and proliferation of HUVECs were inhibited by sodium arsenite in a dose-dependent manner.The cellular mRNA levels of focal adhesion kinase（FAK） and c-Myc were decreased following exposuring to sodium arsenite.Conclusion Sodium arsenite inhibits the migration and proliferation of HUVECs through the downregulation of FAK and c-Myc expressions,suggesting their important roles in reparing the injured vascular endothelial calls.

关 键 词：亚砷酸钠 人脐静脉内皮细胞 黏着斑激酶 原癌基因蛋白 

分 类 号：R361[医药卫生—病理学]