亚砷酸钠选择性诱导G_2+M期NB4细胞凋亡  

Sodium arsenite selectively inducing apoptosis of G2+M phase NB4 cells

作　　者：马东初[1] 孙英慧[1] 马小锋[1] 常奎忠[1] 黄世林 白月辉 初俊杰[1] 刘亚革[1] 

机构地区：[1]沈阳军区总医院医学实验科

出　　处：《中华血液学杂志》1999年第6期296-299,共4页Chinese Journal of Hematology

摘　　要：目的探讨亚砷酸钠诱导ＮＢ４细胞凋亡的作用机制。方法采用流式细胞术、ＤＮＡ电泳和免疫印迹法研究亚砷酸钠对ＮＢ４细胞的作用。结果①亚砷酸钠作用的ＮＢ４细胞先阻滞在Ｇ２＋Ｍ期，而后发生凋亡；②ｄＵＴＰＦＩＴＣ特异性地标记Ｇ２＋Ｍ期ＮＢ４细胞，而且发生在Ｇ２＋Ｍ期阻滞后；③亚砷酸钠处理ＮＢ４细胞，周期蛋白Ａ、Ｅ、Ｄ１、Ｄ２和Ｄ３无明显变化，周期蛋白Ｂ１表达随作用时间的增加而增加；④流式细胞仪分析显示在亚砷酸钠处理的早期（１６ｈ），有部分Ｓ期细胞表达周期蛋白Ｂ１，晚期大部分高表达周期蛋白Ｂ１的细胞处于Ｇ２＋Ｍ期，有少量亚二倍体细胞高表达周期蛋白Ｂ１。结论结果提示亚砷酸钠选择性诱导Ｇ２＋Ｍ期ＮＢ４细胞凋亡时，伴周期蛋白Ｂ１表达的升高。Objective To explore the mechanism of sodium arsenite inducing apoptosis of NB4 cells. Methods The apoptosis of NB4 cells was studied by flow cytometry, DNA gel electrophoresis and Western blot analysis. Results Sodium arsenite induced apoptosis of NB4 cells while the cells were arrested in G2+M phase; dUTPFITC specifically labeled NB4 cells in G2+M phase after the cells were treated with sodium arsenite; After the treatment the expressions of cyclin A, E,D1,D2 and D3 of the NB4 cells did not change significantly, while the expression of cyclin B1 was upregulated with prolongation of the treatment; Some of S phase NB4 cells expressed high level cyclin B1 during the early period of treatment (16h), but most of NB4 cells expressing high level of cyclin B1 were in G2+M phase. ConclusionTBZSodium arsenite selectively induced apoptosis of G2+M phase NB4 cells, accompanied by upregulation of cyclin B1.

关 键 词：NB4 亚砷酸钠 周期蛋白B1 细胞凋亡 白血病 

分 类 号：R733.705[医药卫生—肿瘤]