雷公藤内酯醇对阿尔茨海默病模型大鼠海马补体C1q表达的影响  

Effect of triptolide on expression of C1q in the hippocampus of Alzheimer's disease rats

作　　者：杨宝林[1,2] 吕诚[1] 胡小令[1] 万斌[1] 蔡维君[2] 黄涛波[1] 桂婷[1] 

机构地区：[1]南昌大学医学院人体解剖学教研室,南昌330006 [2]中南大学湘雅医学院人体解剖学与神经生物学系,长沙410013

出　　处：《解剖学报》2010年第6期805-808,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30660073);江西省教育厅科研基金资助项目(赣教技字[2007]87号)

摘　　要：目的探讨雷公藤内酯醇(TP)对阿尔茨海默病模型大鼠海马组织补体C1q表达的影响。方法 30只健康雄性SD大鼠随机分为对照组、模型组及用药组,每组10只。模型组大鼠双侧海马一次性注射凝聚态Aβ1-40,Morris水迷宫检测判断模型成功与否;对照组大鼠海马注射等量生理盐水;用药组大鼠在模型制作成功后每日腹腔注射雷公藤内酯醇0.4mg/kg,共15d。采用免疫组织化学染色和RT-PCR分别检测C1q蛋白和C1q mRNA在大鼠海马组织的表达。结果免疫组织化学染色显示,模型组大鼠海马C1q阳性细胞数(67.89±9.22)较对照组(36.27±5.09)明显增多(P<0.01),平均吸光度(0.2918±0.0347)较对照组(0.1980±0.0183)明显增加(P<0.01);用药组大鼠海马C1q阳性细胞平均吸光度(0.2514±0.0170)较模型组明显减少(P<0.05)。RT-PCR结果显示,模型组大鼠海马C1q mRNA水平(0.722±0.048)明显高于对照组(0.583±0.072)(P<0.01);用药组大鼠海马C1q mRNA水平(0.656±0.053)较模型组明显下降(P<0.01)。结论雷公藤内酯醇可抑制阿尔茨海默病模型大鼠海马组织C1q的表达。Objective To investigate the effect of triptolide （TP） on expression of C lq in the hippoeampus of model rats with Alzheimer＇ s disease（AD）. Methods Thirty male SD rats were randomly divided into control group, AD model group and TP-treated group. Each group had 10 rats. The AD model group was established with bilateral microinjection of aggregated beta-amyloid protein（Aβ） 1-40into hippocampus in rats and Morris water maze test was used to judge that the model was successful or not. The control group was established with bilateral microinjection of normal saline into hippocampus in rats. The TP-treated group rats were administered TP [0. 4mg/（ kg. d） ] intraperitoneally after the AD model was made successfully. The change of expression of C1 q protein and mRNA were observed with immunohistochemical staining and RT-PCR 15 days later. Results Immunohistochemically, the cell number of Clq positive staining in hippocampus of the AD model group （ 67.89±9.22 ） increased obviously as compared with the control group （ 36.27±5.09 ） （ P 〈 0.01 ） , and their average optical density（0. 2918±0. 0347 ） increased obviously as compared with the control group（0. 1980±0. 0183 ） （P 〈 0. 01 ）, the average optical density of Cl q positive staining in hippocampus of the TP-treated group（0.2514±0. 0170） decreased obviously as compared with the A D model group （P 〈 0. 05 ）. In the experiment of RTPCR,the expression of Clq mRNA in hippocampus of the AD model group （0. 722 ± 0. 048 ） increased obviously as compared with the control group（0. 583± 0. 072） （ P 〈 0.01 ）. The expression of Clq mRNA in hippocampus of the TP-treated group（0. 656±0. 053） decreased obviously as compared with the AD model group （ P 〈 0. 01 ）. Conclusion TP can inhibit Clq expression in hippocampus of AD model rats.

关 键 词：雷公藤内酯醇 阿尔茨海默病 C1Q 海马 免疫组织化学 反转录-聚合酶链式反应 大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]