大黄素对人胃癌BGC-823细胞体外增殖和迁移的影响  

Effects of aloe-emodin on proliferation and migration of human gastric cancer cell line BGC-823

作　　者：覃红斌[1,2] 竹梦[3,2] 杜田锴[3,2] 佘乾艳[2,4] 杨吉安[2] 朱晓玲[3,2] 魏蕾[2] 张京伟[3] 

机构地区：[1]湖北民族学院医学院组织学胚胎学教研室,湖北恩施445000 [2]武汉大学基础医学院病理教研室 [3]武汉大学中南医院肿瘤科 [4]武汉大学人民医院肿瘤科,武汉430071

出　　处：《解剖学报》2010年第6期909-911,共3页Acta Anatomica Sinica

基　　金：湖北省自然科学基金资助项目(2008CDB222)

摘　　要：目的探讨大黄素对人胃癌BGC-823细胞增殖和迁移的影响。方法采用四甲基偶氮唑盐(MTT)比色法测定不同浓度大黄素对体外培养人胃癌BGC-823细胞增殖的影响;采用流式细胞术测定大黄素对人胃癌BGC-823细胞的细胞周期影响;采用划痕损伤实验测定大黄素对人胃癌BGC-823细胞迁移的影响。结果 MTT法显示,大黄素能抑制人胃癌BGC-823细胞増殖,呈现浓度依赖性;流式细胞术显示,人胃癌BGC-823细胞的细胞周期主要停滞在G0/G1期;划痕损伤实验表明,人胃癌BGC-823细胞迁移受到明显的抑制。结论大黄素对人胃癌BGC-823细胞的增殖和迁移具有明显的抑制作用,且呈浓度依赖性,并可使人胃癌BGC-823细胞增殖周期停滞于G0/G1期。Objective To investigate the effect of aloe-emodin on proliferation and migration of human gastric cancer cell line BGC-823. Methods The effects of aloe-emodin on cell proliferation,cell cycle stage and migration ability of BGC-823 ceils were observed. The proliferation level was measured by MTT method, cell cycle stage was detected with flow cytometry and migration ability was tested by wound healing assay. Results MTT assay showed the inhibitory effect of aloc-emodin on proliferation of BGC-823 cells was dose-dependent; Treatment of BGC-823 ceils with aloe-emodin resulted in cell cycle arrest at G0/Gl phase by flow cytometry analysis; The migration velocity of BGC-823 cells was obviously inhibited as shown in wound healing assay. Conclusion The inhibitory effect of aloe-emodin on proliferation and migration of BGC-823 ceils was dose-dependent, as well as the cell cycle was arrested at Go/Gj phase, which offers the clue for further understanding the inhibition mechanism of aloe-emodin on human gastric cancer cell line BGC-823.

关 键 词：BGC-82细胞 大黄素 增殖 迁移 四甲基偶氮唑盐检测 人 

分 类 号：R735.2[医药卫生—肿瘤]