阿司匹林联合氟西汀对CUMS抑郁模型大鼠行为学及海马PKA表达的影响  

Effects of aspirin and fluoxetine on the behaviors and hippocampal and cortical expressions of PKA in rats with chronic unpredictable mild stress-induced depression

作　　者：周冉[1] 徐维平[1,2] 王伟迪[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所抗炎免疫药理学省部共建教育部重点实验室抗炎免疫药物安徽省工程技术研究中心,合肥230032 [2]安徽医科大学附属省立医院药剂科,合肥230001

出　　处：《安徽医科大学学报》2011年第1期40-43,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省2008年人才开发专项基金(编号:2008Z049)

摘　　要：目的通过观察阿司匹林联合氟西汀对慢性轻度不可预见性应激(CUMS)大鼠行为学及海马蛋白激酶A(PKA)表达的影响,探讨阿司匹林联合氟西汀的抗抑郁效果。方法 56只SD大鼠,随机分为7组:正常组(A组)、模型组(B组)、大剂量氟西汀联合阿司匹林用药组(C组)、大剂量氟西汀组(D组)、小剂量氟西汀联合阿司匹林用药组(E组)、小剂量氟西汀组(F组)、阿司匹林组(G组)。采用不同应激因子交替持续应激21d,复制大鼠CUMS抑郁模型,观察大鼠行为学和学习记忆能力的变化,用免疫组化法测定海马PKA的表达。结果 C组较之D组,体重增长值及海马PKA表达均增加,学习记忆能力增强;减少氟西汀剂量,联合阿司匹林用药,其抗抑郁效果与大剂量氟西汀相当,体重增长值及糖水摄入量差异均无统计学意义。结论阿司匹林与氟西汀联合应用,减少了氟西汀的毒副反应,抑制了抑郁症中的炎症反应,具有较好的抗抑郁效应。Objective To explore the anti-depressive effect of aspirin and fluoxetine by observing the behaviors and expressions of PKA in the hippoeampus of rats with chronic unpredictable mild stress-induced depression. Methods Fifty-six SD rats were derided into seven groups：normal control （group A）, depression model （group B）, largedose of fluoxetine and aspirin group（ group C ）, large-dose of fluoxetine group （group D） , low-dose of fluoxetine and aspirin group （group E ）, low-dose of fluoxetine group （group F）, aspirin group （group G）. Rats were exposed to ailernate stressors for 21 days to induce depression. Behavioral indexes and changes in learning and memory were observed during the experiments. The expression level of PKA in the hippocampus was determined by immunohistochemistry. Results Compared with group D,weight gain and expression of PKA in hippoeampus and the ability of learning and memory increased in group C;Deereased the dose of fluoxetine combined with aspirin group,the antidepressive effect was correspondent with large-dose of fluoxetine group（ Weight gain and consumption of sugar water had no difference in statistics）. Conclusion That combination of aspirin and fluoxetine can decrease the adverse effects of fluoxetine and inhibite the inflammation reaction in depression has a better anti-depressive effect.

关 键 词：抑郁症/药物疗法 阿司匹林/治疗应用 氟西汀/治疗应用 环AMP依赖性蛋白激酶类 

分 类 号：R749.42[医药卫生—神经病学与精神病学] R971.43[医药卫生—临床医学]