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作 者:张斌[1] 张大鹏[1] 章文斌[1] 王克敏[1] 李冠[1] 姚雅桢[1] 吴建国[1] 石春海[1]
机构地区:[1]浙江大学农业与生物技术学院农学系,浙江杭州310029
出 处:《光谱学与光谱分析》2011年第1期73-76,共4页Spectroscopy and Spectral Analysis
基 金:国家自然科学基金项目(30500319);浙江省科技厅科技攻关项目(2007C12902);浙江省自然科学基金项目(Y3080217)资助
摘 要:色氨酸是人类一种必需氨基酸,也是稻米中一种重要的限制性氨基酸。从4年份1256份材料中选择出272份有代表性的样品,采用碱水解-分光光度法测定了其色氨酸含量。比较不同定标方法的预测结果发现,运用改良的偏最小二乘法(modified partial least square,MPLS)的全局(Global)定标方法和局部(Lo-cal)飞速定标方法的预测效果较佳,基于精米粉光谱建立的方程的预测标准误差均为0.007%,外部验证决定系数分别为87.1%和87.4%,可用于定量分析;而基于糙米光谱建立的定标方程的预测效果略差,但仍具有良好的预测能力。研究结果表明,近红外光谱技术可作为水稻育种中间材料的快速筛选和食品工业中稻米原料的品质监控手段。Tryptophan is one of the essential amino acids in human,and also considered as an important limiting amino acid in rice.Alkali hydrolysis method was used to determine the tryptophan content of 272 representative samples selected from the 1 256 samples in four years.Based on the milled rice flour spectroscopy,by comparing the prediction abilities of the equations with global calibrations and local calibration,the global calibration method with MPLS and the local calibration method were better than others.Their calibration models could be introduced to application because of low standard errors of prediction for both methods(0.007%) and high coefficients of determination(87.1% and 87.4%,respectively).Furthermore,the prediction ability of the equation based on the brown rice spectroscopy could be still used for determination application though their effects were worse than that of the milled rice flour spectroscopy.It was concluded that the equation could be directly used to estimate the content of tryptophan in milled rice,and the technology of near infrared reflectance spectroscopy developed in this study could be applied in selecting breeding materials for higher tryptophan content and in quality control during rice processing.
关 键 词:精米 色氨酸含量 近红外漫反射光谱 全局定标 局部飞速定标
分 类 号:S132[农业科学—农业基础科学]
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