基于不同地理种源头花蓼中没食子酸的含量分析  

Quality Evaluation and Analysis of Polygonum capitatum Based on Different Germplasm Resources

作　　者：周涛[1] 艾强[1] 王彦君[1] 江维克[1] 金艳蕾[1] 魏升华[1] 

机构地区：[1]贵阳中医学院实验中心,贵阳550002

出　　处：《中国实验方剂学杂志》2011年第1期49-52,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科技厅中药现代化项目(黔科合社字[2009]5029);贵州省科技重大专项计划(黔科合重大专项字20080620);贵州省教育厅自然科学研究项目(黔教科2008026)

摘　　要：目的:建立HPLC测定头花蓼中没食子酸含量方法,测定评价48个不同地理种源样品。方法:反相高效液相色谱法,XB-18 C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸溶液(4∶96),流速0.8 mL.min-1,柱温30℃,检测波长220 nm,分别测定头花蓼48个不同地理种源229株样本,SPSS17.0分析结果。结果:没食子酸线性范围进样量在10.24～102.40 ng,r=0.999 9,平均回收率97.32%,RSD 0.52%。所测定样本单株没食子酸含量0.594 6%～0.147 5%,居群间含量0.131 3%～0.330 6%。结论:该方法可准确测定头花蓼中没食子酸含量。在头花蓼不同地理种源中,野生居群间和居群内的没食子酸含量差异较大。Objective：To establish a simple, accurate method of HPLC to determine the gallic acid content of Polygonum capitatum, and to analysis 48 populations in different germplasm resources. Method： The method was developed with the condition as follows：XB-18 Cls （4.6 mm × 250 mm, 5 μm） , acetonitrile-0.4% phosphoric acid（4： 96） as the mobile phase with the speed of 0.8 mL · min-1 and the detection wavelength was 220 nm. Gallic acid was detected in 48 populations with 229 samples. Result： The linear range of gallic acid was 10.24-102.40 ng （r = 0. 999 9） with the average recoveries of 97.32% , RSD 0.52%. The gallic acid content was 0. 594 6%- 0. 147 5% in every sample, and was 0. 131 3%-0. 330 6% during 52 populations. Conclusion： The method is simple, specific, and suitable for the determination of the gallic acid content in P. capitatum. There are some differences in gallic acid contents during populations and whithin populations, especially, it was significant differences in some populations, such as Yunnan-tengeong, Guizhou-taijiang, Guizhou-nayong, Guizhou-yuqing, Guizhou-qinglong and Guizhou-bijie. It had higher contents of gallic acid in Taijiang-laodun, Nayong-yangchang, Yuqing-baini, Shibing-niudaehang populations, Guizhou province, and they should continue to study as suitable growth areas for artificial cultivation of P. capitatum.

关 键 词：头花蓼 地理种源 没食子酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]