γ-羟基丁酸受体与恩氟烷、异氟烷镇痛、催眠作用的关系  

Relationship between gamma-hydroxybutyric acid receptors and the hypnotic and analgesic effects of isoflurane and enflurane

作　　者：周美艳[1] 张明阳[1] 刘亚君[1] 孟晶[2] 戴体俊[2] 杭黎华[3] 邵东华[3] 

机构地区：[1]徐州医学院药理学08级研究生 [2]徐州医学院麻醉药理学教研室,江苏徐州221000 [3]镇江市第一人民医院麻醉科,江苏镇江212001

出　　处：《中国药理学通报》2010年第12期1568-1571,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No30872432,30471657,39970715);江苏省自然科学基金资助课题(NoBK2001143);徐州医学院研究生科技创新计划(NoXY-CX200916)

摘　　要：目的探讨γ-羟基丁酸(gamma-hydroxybutyric acid,GHBA)受体与吸入麻醉药异氟烷和恩氟烷催眠、镇痛作用的关系。方法建立小鼠腹腔和皮下注射吸入麻醉药催眠、镇痛模型。在催醒、热板和扭体实验中分别观察侧脑室(intracerebroventricular,icv)或鞘内(intrathecal,it)注射不同剂量GHBA受体拮抗剂NCS-382对小鼠睡眠时间(sleepingtime,ST)、热板疼痛指数(pain index in hot-plate test,HPPI)和扭体次数(writhing times)的影响。结果催醒实验中,NCS-3821、5、25μg icv均可使异氟烷和恩氟烷催眠小鼠的ST缩短(P<0.01);热板实验中,NCS-3821、5、25μg it对清醒和镇痛小鼠的HPPI没有影响(P>0.05);扭体实验中,皮下注射异氟烷和恩氟烷后引起小鼠的扭体次数减少(P<0.01),但NCS-3821、5、25μg it对清醒小鼠及镇痛小鼠的扭体次数均无明显影响(P>0.05)。结论γ-羟基丁酸受体是吸入麻醉药异氟烷和恩氟烷催眠作用的靶位之一,但与其抗热刺激伤害和抗化学内脏痛作用关系不大。Aim To investigate the relationship between gamma-hydroxybutyric acid receptors（GHBAR）with the hypnotic and analgesic effects of isoflurane and enflurane.Methods Establishing the mice model of hypnosis and analgesia by intraperitoneally injection（ip）and subcutaneously（sc）of appropriate doses of inhalation anesthetics,the different doses of NCS-382 were injected intracerebroventricularly（icv）or intrathecally（it）and then their effects were observed on the sleeping time（ST）,the pain index in hot-plate test（HPPI）and writhing times by using acetic acid-induced writhing test.Results In awaken test,NCS-382 1,5,25 μg（icv）could significantly decrease ST of the mice treated with isoflurane and enflurane（P〉0.01）;in hot-plate test,NCS-382 1,5,25 μg（it）did not affect the HPPI in conscious and treated with isoflurane and enflurane mice（P〈0.05）.In acetic acid-induced writhing test,writhing times inhibition induced by sc administered isoflurane and enflurane was not affected by the NCS-382 1,5,25 μg（P〉0.05）.Conclusion NCS-382 may be one of the important targets for the hypnotic effects on thermal-induced nociception,but not analgesic and chemical-induced nociception of isoflurane and enflurane.

关 键 词：Γ-羟基丁酸受体 异氟烷 恩氟烷 催眠 镇痛 机制 

分 类 号：R-332[医药卫生] R329.11