分光光度法与荧光法测定肝组织丙二醛含量的比较研究  

A comparative study of the determinations of liver malondialdehyde content by spectrophotometry and fluorospectrophotometry

作　　者：刘彬[1] 齐云[1] 李蒙[1] 高源[1] 陈曦[1] 王峥涛[2] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]上海中医药大学中药研究所,上海201203

出　　处：《中国药理学通报》2010年第12期1674-1677,共4页Chinese Pharmacological Bulletin

基　　金：国家科技重大专项课题之"与药效相关的中药质量评价关键技术研究"(No200909502-021);"中药有害残物的检测与分析关键技术研究"(No2009ZX09502-025)

摘　　要：目的比较分光光度法与荧光法测定肝组织MDA的结果,探讨最适合测定肝组织MDA含量的方法。方法通过两种方法所建立的标准曲线计算大鼠肝组织中MDA含量及体外FeSO4诱导肝匀浆生成的MDA量,采用荧光法测定灯盏花素体外对FeSO4诱导肝组织生成MDA含量的影响。结果分光光度法测定肝组织MDA含量及体外FeSO4诱导MDA含量的△A值均在标准曲线最低测定值0.1以下,而荧光法测定值均在其标准曲线最低测定值0.4以上。灯盏花素终浓度在1.02～11.25mg·mL-1可抑制FeSO4诱导的肝组织生成MDA,且有一定的量效关系。结论由于肝匀浆本底的干扰,采用分光光度法往往难于通过其标准曲线计算出MDA准确含量,不适合用于肝组织的测定。建议采用荧光法替代。Aim By comparing the results of the determinations of liver malondialdehyde（MDA）content by spectrophotometry and fluorospectrophotometry,we hope to find a better appropriate method for determining liver MDA content.Method In this study,we determined the MDA content in rat liver and the content of liver MDA that was induced by FeSO4 in vitro by spectrophotometry and fluorospectrophotometry respectively.And we also determined the effect of breviscapine in inhibiting the content of liver MDA induced by FeSO4 in vitro by fluorospectrophotometry.Result △A values determined by spectrophotometry were all lower than 0.1;△A values determined by fluorospectrophotometry were all higher than 0.4.The inhibition effects of breviscapine（1.02-11.25 mg·L^-1）in the production of MDA that induced by FeSO4 in vitro were detected by fluorospectrophotometry,and it exhibited a certain dose-effect relationship.Conclution Due to the high background level of liver homogenates,it was diffcult to calculate the MDA content with spectrophotometry.Fluorospectrophotometry was a better method for determining liver MDA content.

关 键 词：脂质过氧化 肝组织 丙二醛(MDA) 分光光度法 荧光法 灯盏花素 

分 类 号：R-332[医药卫生] R322.47