吡那地尔对缺血缺氧PC12细胞凋亡及p-CREB信号通路的影响  

Effect of pinacidil on the apoptosis and the PI3K/Akt/CREB signal pathway of PC12 cells induced by ischemia and hypoxia

作　　者：鲁杨[1] 张鸿[1] 马英[1] 

机构地区：[1]中国医科大学盛京医院神经内科,辽宁沈阳110004

出　　处：《解剖科学进展》2010年第6期527-530,共4页Progress of Anatomical Sciences

摘　　要：目的观察KATP通道开放剂吡那地尔对缺血缺氧PC12细胞凋亡的影响,探讨KATP通道开放剂对缺血缺氧PC12细胞凋亡的信号转导机制。方法取传代后3d的PC12细胞,分为正常对照组、缺血对照组、吡那地尔预处理组、吡那地尔+格列吡嗪处理组共4组。采用Annexin-VFITC/PI双染流式细胞分析仪检测凋亡率,应用Western-blotting检测p-CREB蛋白表达水平,观察KATP开放剂对缺血缺氧PC12细胞凋亡的保护作用。结果与缺血对照组相比,吡那地尔预处理组PC12细胞凋亡率明显降低(p<0.05),吡那地尔预处理组PC12细胞p-CREB蛋白表达增加(p<0.05)。结论 KATP通道开放剂吡那地尔能明显降低缺血缺氧后PC12细胞凋亡,增加p-CREB蛋白表达,CREB可能是KATP通道开放剂减少缺血缺氧后PC12细胞凋亡的途径之一。Objective To observe the effect and mechanism of KATP channel opener（pinacidil） on the apoptosis and the PI3K/Akt/CREB signal pathway of PC12 cells induced by ischemia and hypoxia. Methods PC12 cells cultured 3d after passage were divided into： normal control group, ischemic and hypoxia group, pinacidil treatment, pinacidil＋glipizide group. Neuronal apoptosis was detected by Annexin-V FITC/PI double-dyed flow cytometry, the expression of p-CREB protein was detected by Western-blotting. Results Compared with ischemic and hypoxia group, the apoptosis rate of PC12 cells decreased（P〈0.05） and the expression level of p-CREB protein in PC12 cells increased （P〈0.05） in pinacidil treated group. Conclusion The decrease of PC12 cells apoptosis and increase of p-CREB protein expression by pinacidil indicate that CREB might be related to the inhibitory mechanism of apoptosis in hypoxia-ischemia PC12 cells by pinacidil.

关 键 词：吡那地尔 ATP敏感性钾通道 PC12细胞 凋亡 P-CREB 

分 类 号：R743.31[医药卫生—神经病学与精神病学]