急性髓系白血病患者dapk基因启动子甲基化改变的研究  

Alteration of Methylation Status of Death-associated Protein Kinase (dapk) Gene Promoter in Patients with Acute Myeloid Leukemia

作　　者：钱军[1] 姚冬明[1] 林江[2] 陈芹[1] 李云[1] 纪润壁[1] 杨静[1] 钱震[1] 肖高飞[2] 王雅丽[1] 

机构地区：[1]江苏大学附属人民医院血液科,江苏镇江212002 [2]江苏大学附属人民医院中心实验室,江苏镇江212002

出　　处：《中国实验血液学杂志》2010年第6期1390-1394,共5页Journal of Experimental Hematology

基　　金：江苏省医学重点人才资助项目(编号RC2007035);江苏省卫生厅‘科教兴卫工程’-苏州大学附属第一医院血液学学科开放课题基金(编号KF200944);江苏大学临床医学科技发展基金资助项目(编号JLY20080048);镇江市社会发展项目(编号SH2006032)

摘　　要：本研究旨在分析中国人群急性髓系白血病(acute myeloid leukemia,AML)患者死亡相关蛋白激酶(deathassociated protein kinase,DAPK)基因启动子的甲基化状况及其临床相关性。应用甲基化特异性PCR(methylation-specific PCR,MSP)技术对112例AML患者骨髓标本dapk基因甲基化状态进行检测。结果表明:13例对照组均未发生dapk基因启动子甲基化,而112例AML患者中82例(73.2%)发生dapk基因甲基化,dapk基因甲基化改变与患者的性别、年龄、白细胞、血红蛋白、血小板计数、AML分型以及染色体异常等结果均无相关性(p>0.05)。结论:dapk基因甲基化是AML中的一个常见分子事件。This study was purposed to analyze the methylation status of death-associated protein kinase （dapk） gene promoter in Chinese patients with acute myeloid leukemia （AML） and its relationship with clinical features. The methylation-specific PCR （MSP） technique was used to detect dapk promoter methylation in bone marrow samples from 112 cases of AML. The results indicated that gene dapk promoter hypermethylation was detected in 82 cases （73.2%）, but not in 13 control group. There was no correlation of dapk gene hypermethylation with sex, age, WBC counts, platelet counts, hematologic parameters, chromosomal abnormalities and different subtypes of AML patients. It is concluded that dapk gene hypermethylation may be a common molecular event in AML.

关 键 词：DAPK基因 急性髓系白血病 DNA甲基化 

分 类 号：R733.71[医药卫生—肿瘤]