乙酰基-11-酮基-β乳香酸对急性髓系白血病细胞HL-60细胞增殖、凋亡和细胞周期的影响  

Effect of Acetyl-11-Keto-β-Boswellic Acid on Proliferation,Apoptosis and Cell Cycle of Human Acute Myeloid Leukemia Cell Line HL-60

作　　者：袁小瑜[1] 李跃辉[2] 齐振华[1] 彭敏源[1] 万智[1] 王光平[1] 陈方平[1] 

机构地区：[1]中南大学湘雅医院血液科,湖南长沙410008 [2]中南大学肿瘤研究所,湖南长沙410078

出　　处：《中国实验血液学杂志》2010年第6期1440-1444,共5页Journal of Experimental Hematology

基　　金：湖南省自然科学基金(编号07JJ3073);湖南省卫生厅科研项目(编号B2007022)

摘　　要：本研究旨在探讨乙酰基-11-酮基-β乳香酸(3-O-acetyl-11-keto-β-boswellic acid,AKBA)对人髓系白血病细胞株HL-60细胞生长、增殖、凋亡及细胞周期的影响。用不同浓度AKBA处理对数生长期HL-60细胞;采用MTT法评价AKBA对HL-60细胞株生长的抑制作用;采用Hochest33342染色观察细胞的形态学变化;Annexin-V-FITC/Propidium Iodide(PI)双染法结合流式细胞术检测细胞凋亡率;采用PI单标法结合流式细胞术检测AKBA对HL-60细胞周期进程的影响。结果表明:AKBA对HL-60细胞增殖有抑制作用。当AKBA浓度增大时,AKBA诱导的细胞凋亡效应也有所增强。AKBA使细胞周期发生变化,导致S期细胞减少,G1期细胞增多。结论:AKBA对HL-60细胞具有抗增殖和诱导细胞凋亡的作用,在急性髓系白血病治疗领域有良好前景。The aim of this study was to investigate the effect of 3-O-acetyl-11-keto-β-boswellic acid（AKBA） on the proliferation,apoptosis and cell cycle of human acute myeloid leukemia （AML） cell line HL-60. HL-60 cells were treated by AKBA at various concentrations. The inhibitory effects of AKBA on the proliferation of HL-60 were analyzed by MTT assay. Morphologic changes of HL-60 cells were observed by fluorescence microscopy with Hochest33342 staining. Cell apoptosis rate was determined by flow cytometry with Annexin-V-FITC/PI double staining. The cell cycle was measured by flow cytometry with PI staining. The results showed that AKBA inhibited the proliferation of HL-60 and the apoptosis rate of HL-60 cells was gradually enhanced when AKBA dose increased. AKBA changed the cell cycle of HL-60,resulting in cell increase at G1 phase and dicrease at S phase. It is concluded that the AKBA has anti-proliferation and apoptosis-inducing effects on HL-60 cells,that seems a promising therapeutical approach for AML.

关 键 词：乳香酸 白血病 HL-60细胞 细胞凋亡 细胞增殖 细胞周期 

分 类 号：R733.1[医药卫生—肿瘤] R989.1[医药卫生—临床医学]