两株产人参皂苷糖苷酶酵母菌株的18S rDNA和ITS序列分析  

Structure and Sequence Analysis of 18s rDNA and ITS Gene of Two Yeast Strains Producing Ginsenoside Glycosidases

作　　者：李东霄[1] 常景玲[1] 张志宏[1] 王改霞[1] 

机构地区：[1]河南科技学院生命科技学院,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2010年第6期128-131,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省教育厅自然科学研究计划项目(2009B180006);校博士启动资金资助

摘　　要：对两株产人参皂苷糖苷酶的酵母菌株进行核糖体18S rDNA和ITS序列克隆测定,获得了长度分别为1 477和1 478 bp的18S rDNA序列和长度分别为791和727 bp的ITS序列,对获得的基因序列进行比对及同源性分析,结果显示,两株酵母菌的18S rDNA序列的相似性达100%,而ITS序列的相似性则为66%,均与NCBI数据库中登录的啤酒酵母的相应序列同源性最高.从GenBank中选取部分不同种属的酵母菌ITS序列,以ITS为对象构建系统发育树,从分子生物学角度确定了两株酵母菌为酵母菌属的不同种类.The sequences of 18S rDNA and ITS gene fragments of two yeast strains which can produce ginsenoside glycosidases are cloned and analyzed,and both 18S rDNA and ITS gene sequences are obtained.It is shown from the homologous analysis results that the sequence similarity of 18S rDNA and ITS fragments from two yeast strains is 100% and 66% respectively,and the 18S rDNA and ITS gene sequences are highly homologous to the relevant sequences of Saccharomyces cerevisiae in GenBank.The ITS sequences of 10 yeast species which belong to different genera are retrieved from GenBank,and the phylogenetic tree is then constructed by using these sequences and neighbor-joining method.The results from molecular biological analysis show that these two yeast strains are identified as different species of Saccharomyces.

关 键 词：酵母菌 18S rDNA ITS序列 分子分类 

分 类 号：Q78[生物学—分子生物学]