茶多酚对小鼠小肠黏膜组织结构及5-羟色胺分泌的影响  

Effects of Tea Polyphenols on Mucosa Structures and 5-Hydroxytryptamin Secretion of Small Intestine in Mouse

作　　者：唐超智[1] 贠小静[1] 张亚捷[1] 杨海[1] 孙锦霞[1] 张文学[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2010年第6期139-141,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金青年基金(51008119);河南师范大学青年科学基金;河南师范大学大学生创新性实验基金资助;河南省动物学重点学科基金

摘　　要：目的:研究茶多酚(TP)对小鼠小肠黏膜组织结构及5-羟色胺(5-HT)分泌的影响.方法:昆明种小鼠28只,随机均分为A,B,C,D组.A组为对照组,以蒸馏水灌胃,剂量为0.20 mL/d;B,C,D组以茶多酚灌胃,剂量分别为0.50、1.00、2.00 mg/d,连续灌胃30 d.于第31 d取小鼠血清放免测定5-HT含量;取小鼠小肠以甲醛固定后制作石蜡切片,H-E染色示肠黏膜组织结构变化,免疫组化染色示肠黏膜内5-HT分泌细胞数量变化.结果:与A组相比,B,C,D组小鼠血液5-HT含量较低,小肠绒毛疏松,肠腺萎缩,黏膜内5-HT分泌细胞数量明显较少.结论:连续服用TP对小鼠小肠黏膜组织结构和5-HT分泌均有抑制性影响.Objective The paper studies the effects of tea polyphenols（TP） on mucosa structures and 5-hydroxytryptamin（5-HT） secretion of small intestine in mouse.Methods Twenty-eight Kunming mice were classified randomly into four groups： A,B,C and D,every group had 7 mice.Group A was administered intragastrically with 0.20 ml/d deionized water for 30 days.Respectively,Group B,C and D were administered intragastrically with 0.50,1.00,2.00 mg/d TP for 30 days.On the 31st day,the research decided the 5-HT content of serum every mouse by radioimmunoassay,and dissected small intestine to fix with formalin,then made paraffin embedded section,detected histological structure of small intestine by H-E dyeing,counted 5-HT endocrine cells of unit area in small intestine by immunohistochemistry technology.The results were compared with Group A,significantly,the serum 5-HT content of Group B,C and D was low,alinement of intestine villus was loose,intestine gland was atrophic,quantity of 5-HT endocrine cells was less.Conclusion Results reveal that TP had an inhibiting effect on hyperblastosis and 5-HT secretion of small intestine in mouse.

关 键 词：茶多酚 小鼠 小肠 5-羟色胺 

分 类 号：Q959.5[生物学—动物学]