肺炎衣原体感染对血管平滑肌细胞黏附和迁移的影响  

Effects of Chlamydia pneumoniae infection on adhesion and migration of vascular smooth muscle cells

作　　者：王蓓蓓[1] 张利军[1] 权伟[1] 沈炳玲[1] 张腾腾[1] 张丽莙[1] 

机构地区：[1]天津医科大学基础医学院病理生理学教研室,天津300070

出　　处：《中国病理生理杂志》2010年第12期2289-2294,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30971225);教育部科学技术研究重点资助项目(No.206008);教育部留学回国人员科研启动基金资助项目(2005年)

摘　　要：目的:观察肺炎衣原体(C.pn)感染对血管平滑肌细胞(VSMCs)黏附和迁移的影响。方法:C.pn在人喉癌细胞系HEp-2细胞中增殖培养后感染大鼠VSMCs,吖啶橙(AO)荧光染色观察细胞内C.pn包涵体的形态特点;聚合酶链反应(PCR)检测C.pn特异性DNA片段;细胞黏附实验观察C.pn感染对VSMCs黏附能力的影响;wound-healing assay和Transwell assay检测C.pn感染VSMCs后细胞迁移能力的改变。结果:C.pn感染VSMCs后,少数细胞内出现空泡状结构即包涵体,但在多数细胞内以点状感染灶形式存在,包涵体较大,但数量较少。利用PCR可在感染的VSMCs内检测到437 bp的C.pn特异性DNA片段。细胞黏附实验结果显示,C.pn感染VSMCs 2 h后,细胞黏附能力明显增强,其吸光度值明显高于正常对照组(P<0.01),细胞黏附率为134.38%。Wound-healing assay结果显示,C.pn感染VSMCs 24 h后,细胞向划痕中央迁移的距离明显大于正常对照组(P<0.05)。Transwell assay结果显示,C.pn感染VSMCs 24 h后,C.pn感染组的细胞迁移数明显多于正常对照组(P<0.01)。结论:C.pn感染能够显著增强VSMCs的黏附和迁移能力。AIM：To investigate the effects of Chlamydia pneumoniae（C.pn） infection on the adhesion and migration of vascular smooth muscle cells（VSMCs）.METHODS： Primary VSMCs,isolated from the aorta of SD rats,was infected with C.pn after the culture and propagation of C.pn in the HEp-2 cells.The morphological characteristics of C.pn inclusions in VSMCs were examined under the fluorescence microscope with acridine orange（AO） staining.The specific DNA fragment of C.pn was identified by polymerase chain reaction（PCR）.Cell adhesion assay was performed to investigate the effect of C.pn infection on the adhesion of VSMCs to collagen I.Wound-healing assay and Transwell assay were performed to detect the effects of C.pn infection on VSMCs migration.RESULTS： The C.pn inclusions and infection spots were observed in VSMCs under fluorescence microscope with AO staining.The inclusions were larger than infection spots in volume,but were much lower in count.A 437 bp-specific fragment of C.pn DNA was detected in C.pn-infected VSMCs by PCR.In the cell adhesion assay,the absorbance values in C.pn infection group were far higher than those in control group 2 h after infection（P0.01）.The cell adhesion ratio in C.pn infection group was 134.38%.The migration distance of VSMCs infected with C.pn was significantly longer than that of the control cells 24 h after infection by a wound-healing assay（P0.05）.More migratory VSMCs infected with C.pn for 24 h were found in a Transwell assay than those in control group（P0.01）.CONCLUSION： C.pn infection significantly promotes the adhesion and migration of VSMCs.

关 键 词：动脉粥样硬化 肺炎衣原体 血管平滑肌细胞 黏附 迁移 

分 类 号：R363[医药卫生—病理学]