参慈胶囊联合顺铂抑制小鼠Lewis肺癌组织血管生成的机制研究  

Inhibitory effect of Shenci capsule in combination with DDP on angiogenesis in mice with Lewis lung cancer

作　　者：徐立群[1,2] 张荣华 薛兴阳[1,2] 邬晓东[1,2] 傅淑平[4] 蔡宇 梁昆[1,2] 陈锐深[5] 

机构地区：[1]广州医学院附属肿瘤医院 [2]广州医学院肿瘤研究所,广东广州510095 [3]暨南大学药学院,广东广州510632 [4]香港大学中医药学院 [5]广州中医药大学第一附属医院,广东广州510405

出　　处：《中国病理生理杂志》2010年第12期2347-2350,共4页Chinese Journal of Pathophysiology

基　　金：广东省中医药管理局资助项目(No.1060078)

摘　　要：目的:探讨参慈胶囊及顺铂对Lewis肺癌组织生长及血管生成的抑制作用。方法:将40只C57BL/6小鼠自接种Lewis肺癌瘤株细胞建立实体瘤模型,随机分为参慈胶囊组、参慈胶囊+顺铂组、顺铂组、模型组。模型组予等量生理盐水,其余各组荷瘤小鼠均用药21 d,测量肿瘤重量并计算抑瘤率,采用免疫组化技术检测血管内皮生长因子(VEGF)、微血管密度(MVD)表达情况;采用实时荧光定量PCR(FQ-PCR)技术检测Lewis肺癌荷瘤小鼠癌细胞表达血管内皮生长因子受体-2(KDR)mRNA的情况。结果:(1)在抑瘤率方面,参慈胶囊+顺铂组抑瘤率明显高于其它3组,差异显著(P<0.01)。(2)参慈胶囊+顺铂组能降低Lewis肺癌组织VEGF、KDR的表达及MVD的形成,差异显著(P<0.01)。结论:(1)参慈胶囊联合顺铂能抑制Lewis肺癌组织生长及血管生成,二者联用具有相加或者协同效应;(2)下调VEGF及其受体KDR的表达,可能是二者联合抗肿瘤血管生成的机制之一。AIM：To investigate the inhibitory effect of Shenci capsule,a Chinese medicine,in combination with cisplatin（DDP） on the tumor growth and angiogenesis in mouse Lewis lung cancer.METHODS： Forty mice with Lewis lung cancer were randomly divided into 4 groups： Shenci capsule group,Shenci capsule in combination with DDP group,DDP group and control group.Shenci capsule,Shenci capsule in combination with DDP,DDP and sodium chloride wene given,respectively.After 21 days,the weight of subcutaneous tumors was measured,and the tumor inhibition rate was calculated.The microvessel density（MVD） and the protein level of vascular endothelial growth factor（VEGF） were detected by immunohistochemical（IHC） staining.The mRNA expression levels of kinase insert domain-containing receptor（KDR） and the receptor of VEGF were detected by FQ-PCR.RESULTS： The tumor growth inhibitory rate in Shenci capsule in combination with DDP group（63.99%） was higher than that in Shenci capsule group,DDP group and control group（P0.01）.The tumor MVD,VEGF protein and KDR mRNA expression level in Shenci capsule in combination with DDP group were lower than those in the above three groups（P0.01）.CONCLUSION： Shenci capsule in combination with DDP have addition or synergistic effects to inhibit the tumor growth and angiogenesis in mouse Lewis lung cancer.Down-regulation of VEGF and its receptor KDR may be one of the mechanisms that combined use of these two drugs for inhibiting angiogenesis.

关 键 词：参慈胶囊 血管生成 微血管密度 

分 类 号：R730.58[医药卫生—肿瘤]