黄嘌呤核苷促进骨髓间质干细胞体外快速扩增的实验研究  

Rapid proliferation of bone marrow mesenchymal stem cells enhanced by xanthosine

作　　者：刘芳[1,2,3] 陈少红[2,3] 刘青波[2,3] 范新娟[2,3] 唐芳[2,3] 刘大伟[2,3] 赵国强[2,3] 

机构地区：[1]南华大学医学院病理学教研室肿瘤细胞与分子病理学湖南省高校重点实验室,湖南衡阳421001 [2]中山大学中山医学院病理学教研室 [3]中山大学附属第一医院病理科,广东广州510080

出　　处：《中国病理生理杂志》2010年第12期2389-2393,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30170473);广东省自然科学基金资助项目(No.04009326);湖南省教育厅课题资助项目(No.08C744);湖南省高校重点实验室经费资助项目(No.09K074;No.09K075)

摘　　要：目的:探讨黄嘌呤核苷(Xs)对骨髓间质干细胞(BMSCs)增殖及分化状态的影响。方法:将Xs加入骨髓间质干细胞培养体系,观察它对骨髓间质干细胞增殖和分化特性的影响。结果:在Xs的影响下,传至第10代的细胞生长速率与第4代的细胞基本保持一致,无下降趋势。而对照组细胞(无Xs影响)传至第10代时,生长速率则有明显下降。经Xs促进增殖的骨髓间质干细胞仍能保持足够的肝向分化能力。结论:Xs作为非平衡细胞动力学分裂方式的抑制剂,既能实现骨髓间质干细胞的分裂方式由非平衡态向平衡态转变,又不影响其肝向分化的特性,有助于提高骨髓间质干细胞的体外增殖效率,有着广泛的应用空间。AIM：To explore the effect of xanthosine（Xs） on proliferation and differentiation of bone marrow mesenchymal stem cells（BMSCs）.METHODS： Xs was directly added to the culture system and the effects of Xs on proliferation and differentiation of BMSCs were observed.RESULTS： In the presence of Xs,the growth rate of the 10th passage cells was almost similar to that of the 4th passage cells and no downtrend was observed.However,in control group（without Xs）,the growth rate of the 10th passage cells was obviously declined.The BMSCs promoted by Xs still kept up a vigorous capability of differentiation into hepatocytes.CONCLUSION： As an inhibitor of asymmetric cell kinetics,Xs can promote the conversion of BMSCs from asymmetric cell kinetics to symmetric cell kinetics,and keeps synchronously the ability of differentiation from BMSCs into hepatocytes as well.It is helpful for enhancing the proliferation efficiency of BMSCs in vitro,and will have extensive application of clinical practice.

关 键 词：黄嘌呤核苷 骨髓间质干细胞 快速扩增 细胞分化 

分 类 号：R394.2[医药卫生—医学遗传学]