黄芪中7种活性成分对缺氧诱导因子(HIF-1)表达的影响  

Effect of 7 Kinds of Active Constituents from Astragalus membranaceus on the Expression of HIF-1

作　　者：梁丽娟[1] 董婷霞[2] 詹华强[2] 屠鹏飞[3] 赵奎君[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京市100050 [2]香港科技大学生物系暨中药研发中心,香港特别行政区999075 [3]北京大学医学部药学院,北京市100083

出　　处：《中国药房》2010年第47期4422-4424,共3页China Pharmacy

基　　金：国家重点基础研究发展计划(973计划)课题(2006CB504707)

摘　　要：目的:研究黄芪中的7个主要活性成分芒柄花素、芒柄花苷、毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、乙酰黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷对缺氧诱导因子(HIF-1)表达的影响。方法:将包含有高度保留的HIF-1结合位点的缺氧应答原件(HRE)插入到含有荧光素酶报告基因的pBI-GL质粒中,磷酸钙沉降法将构建的质粒转染到人胚肾(HEK)293T纤维原细胞。用不同浓度的药物处理48h后测定荧光素酶的活性,通过荧光素酶报告基因系统评估HIF-1的表达。结果:10μmol·L-1芒柄花素可促进HIF-1的表达。芒柄花苷与HIF-1的表达呈浓度-效应依赖关系,低浓度促进其表达,高浓度抑制其表达。其它5种单体与对照比较无明显作用趋势。结论:本试验可为黄芪的抗肿瘤作用及其他疾病的治疗提供一定依据。OBJECTIVE： To explore the effect of formononetin,ononin,calycosin-O-β-D-glucoside,calycosin,acetylastragalosideⅠ,astragalosideⅡ, astragaloside Ⅳ from Astragalus membranaceus on the expression of hypoxia-induced factor-1（HIF-1）. METHODS： The HRE, derived from EPO containing a highly conserved HIF-1 binding site, were cloned into pBI-GL vectors that contained luciferase reporter genes. The HEK-293T cells were transfected cDNA constructs by calcium phosphate precipitation methods. The activity of luciferase was determined after 48 h treatment. The expression of HIF-1 was evaluated using luciferase report gene system. RESULTS：10 μmol·L-1 formononetin promote expression of HIF-1.Ononin had an effect on the expression of HIF-1 in a concentration-dependent manner. Low concentration could elevate the expression of HIF-1 while high concentration down-regulated the expression. Other 5 monomers had no obvious effect on the expression of HIF-1. CONCLUSION：The study provide reference for the anti-tumor effect of A. membranaceus and therapeutic effect of it for other disease.

关 键 词：人胚肾细胞 缺氧诱导因子 黄芪 瞬时转染 荧光素酶报告载体 芒柄花苷 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]