外置式植物纤维床反应器与膜分离联用的丙酸发酵工艺研究  

Propionic Acid Production in an External Plant Fibrous-Bed Bioreactor Combined with Hollow Fiber Membrane

作　　者：陈飞[1] 徐虹[1] 冯小海[1] 吴波[1] 李莎[1] 

机构地区：[1]南京工业大学食品与轻工学院材料化学工程国家重点实验室,江苏南京210009

出　　处：《高校化学工程学报》2010年第6期1000-1004,共5页Journal of Chemical Engineering of Chinese Universities

基　　金：国家"973"项目(2007CB714304);国家自然科学基金(201006050);国家博士后基金(20100471326);江苏省博士后基金(0901011C)

摘　　要：开发了一种外置式植物纤维床反应器与膜分离联用的丙酸发酵装置,将外置式植物纤维床反应器与中空纤维膜组件相连接,即可实现丙酸的多批次稳定高效生产。实验结果表明,以Propionibacterium freudenreichii CCTCC M207015为生产菌株,葡萄糖做碳源,利用该装置发酵214h(5批次),批次平均丙酸浓度达50.77g·L-1,生产效率达1.19g·L-1·h-1,菌体干重最高达33.85g·L-1,丙酸对葡萄糖转化率和丙酸占总有机酸比例分别为50.77%、79.45%(w/w),均远优于游离细胞与膜分离联用的丙酸发酵装置。Using glucose as carbon source and Propionibacterium freudenreichii CCTCC M207015 as the fermentation strain,a hollow fiber membrane combined with a novel external plant fibrous-bed bioreactor（PFB） was constructed and applied for the propionic acid production.The results show that the equipment could be efficient and stable for the repeated-batch propionic acid fermentation.After five batches of fermentation（214 h）,the average batch propionic acid concentration is 50.77 g·L-1 with a productivity of 1.19 g·L-1·h-1,and the maximal dry cell weight obtained is 33.85 g·L-1.Glucose conversion efficiency and the proportion of propionic acid to total organic acids are approximately 50.77% and 79.45%（w/w）,respectively.All of the data are better than that of the propionic acid production using the free cell propionic acid fermentation combined with hollow fiber membrane.

关 键 词：丙酸 外置式植物纤维床反应器 中空纤维膜 多批次发酵 

分 类 号：O658.2[理学—分析化学] TQ921.7[理学—化学]