米曲霉外切β-D-氨基葡萄糖苷酶的化学修饰  

Chemical Modification of Exo-β-D-Glucosaminidase from Aspergillus Oryzae

作　　者：李冬霞[1] 夏文水[2] 

机构地区：[1]苏州农业职业技术学院食品系,江苏苏州215008 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品研究与开发》2010年第12期196-199,共4页Food Research and Development

基　　金：国家自然科学基金项目(20876068)

摘　　要：采用化学修饰剂对来源于米曲霉(Aspergillus Oryzae)的外切β-D-氨基葡萄糖苷酶(GlcNase)进行修饰。氯胺-T(Ch-T)和碳二亚胺(EDAC)的修饰结果表明甲硫氨酸残基和羧基是GlcNase的必需基团。用化学修饰剂对GlcNase的羟基、精氨酸残基、酪氨酸残基、巯基和二硫键进行修饰,结果表明:修饰剂在较高的浓度范围内均没有引起酶活的显著降低,因此羟基、精氨酸残基、酪氨酸残基、巯基和二硫键都不是维持GlcNase酶活的必需基团。Chemical modification of exo-β-D-glucosaminidase from Aspergillus Oryzae was performed.Results of modification by Ch-T and EDAC indicate that methionine residues and carboxyl groups were essential groups of GlcNase.However,hydroxyl groups,arginine residues,tyrosine residues,sulfydryl groups,and disulfide bonds were not essential groups of GlcNase.

关 键 词：β-D-氨基葡萄糖苷酶 化学修饰 必需基团 

分 类 号：Q55[生物学—生物化学]