BDNF对新生鼠缺血缺氧性脑损伤的保护作用的研究  

Protective effect of BDNF to newborn rats with hypoxic-ischemic brain damage

作　　者：徐晓晓[1] 周江堡[1] 

机构地区：[1]重庆医科大学附属儿童医院神经内科,重庆400014

出　　处：《重庆医科大学学报》2010年第11期1642-1645,共4页Journal of Chongqing Medical University

摘　　要：目的:研究提前给予脑源性神经生长因子(Brain-derived neurotrophic factor,BDNF)对新生鼠的缺血缺氧性脑损伤(Hypoxic-ischemic brain damage,HIBD)的保护作用。方法:选取7 d龄Wistar大鼠256只,随机分成4组,①假手术组(n=64,A),②模型组(n=64,B),③模型前给药组(n=64,C),④模型后给药组(n=64,D),每组又分为1、3、5、7 d 4个时间点,每个时间点8只,用免疫组织化学和实时荧光定量PCR方法检测脑组织中海马区域的Trk-B和p75的表达水平。结果:模型前注射组和模型后注射组的Trk-B和p75水平分别与对照组和假手术组比较均有显著性差异(P<0.05)。结论:缺血缺氧模型前给予BDNF可保护HIBD引起的脑损伤,其可能机制是通过提高Trk-B的表达水平和降低p75的表达水平来发挥作用的。Objective：To study the protective effect of BDNF to newborn rats with hypoxic-ischemic brain damage before the modeling.Methods：Two hundred and fifty-six 7-day-old Wistar rats were randomly divided into four groups： sham operation group（n=64,A）;model group（n=64,B）;model pre-treated group（n=64,C）;model post-treated group（n=64,D）,every group are further divided into 1、3、5、 7 d four time points（n=8）.The expression of Trk-B and p75 in Hippocampus was examined with immunohisto chemical staining and real-time PCR.Results：Trk-B and p75 levels in model pre-treated group and model post-treated group,had significant differences compaed with model group and sham operation group,P0.05.Conclusion：BDNF has a protective effect to the brain injury caused by HIBD. Enhancing the expression of Trk-B levels and reduce the expression level of p75 may play a role.

关 键 词：脑缺血 脑缺氧 脑源性神经生长因子 Trk-B P75 

分 类 号：R748[医药卫生—神经病学与精神病学]