多环芳烃降解菌的筛选及其对天然水的净化效果分析  

Isolation of A PAHs-Degrading Bacterium and Analysis of Its PAHs-Degrading Effect in Water

作　　者：魏妍[1] 杨娇艳[1] 王文玲[1] 李腾[1] 杨劭[1] 

机构地区：[1]华中师范大学湖北省城市水环境生态学重点实验室,湖北武汉430079

出　　处：《化学与生物工程》2010年第12期56-60,共5页Chemistry & Bioengineering

基　　金："十一五"水专项东江项目(2008ZX07211-003);湖北省自然科学基金重点资助项目(2008CDA101);武汉市科技计划项目(200760423164)

摘　　要：采用水-硅油双相体系筛选法,从武汉石化厂活性污泥中分离到一株能以多环芳烃(Polycyclic aromatichydrocarbons,PAHs)萘、菲、芴、芘、荧蒽为唯一碳源的高效降解菌株W3,16S rDNA序列分析表明W3属于假单胞菌属(Pseudomonas citronellolis)。在多环芳烃初始浓度为30 mg.L-1的无机盐-硅油培养液中培养5 d后,W3对萘、菲、芴、芘、荧蒽的降解率分别达到(94.00±2.83)%、(81.15±1.63)%、(87.65±4.71)%、(91.65±1.63)%和(87.20±2.12)%。用W3对湖水中的多环芳烃进行降解,结果表明,在投菌量为3%、细菌密度为3.15×106个.mL-1时,5 d后W3对萘、菲、芴、芘、荧蒽的降解率分别达到(34.07±7.07)%、(24.78±0.51)%、(56.95±5.06)%、(19.67±3.18)%、(35.10±5.22)%;当细菌密度为4.76×107个.mL-1时,5 d后W3对萘、菲、芴、芘、荧蒽的降解率分别达到(59.27±8.54)%、(44.35±10.79)%、(66.64±2.35)%、(26.40±6.36)%和(53.86±4.07)%。A high efficiency PAHs-degrading strain W3,which could use naphthalene,phenanthrene,fluorene,pyrene,fluoranthene as the sole carbon and energy source,was isolated from the activated sludge of Wuhan Petrochemical Works by the water-silicon oil biphasic system.W3 was indentified as Pseudomonas citronellolis according to the phylogenetical analysis results of 16S rDNA sequence.In the mineral salts-silicon oil medium under PAHs initial concentration of 30 mg·L^-1,the degradation rate of naphthalene,phenanthrene,fluorene,pyrene,fluoranthene was（94.00±2.83）%,（81.15±1.63）%,（87.65±4.71）%,（91.65±1.63）% and（87.20±2.12）%,respectively after five days.In the research on application of W3 in South Lake water,with inoculation amount of 3% and bacterial density of 3.15×10^6 cfu·mL^-1 by bioaugmentation,five days later,the degradation rate of naphthalene,phenanthrene,fluorene,pyrene,fluoranthene reached（34.07±7.07）%,（24.78±0.51）%,（56.95±5.06）%,（19.67±3.18）% and（35.10±5.22）%,respectively;and bacterial density of 4.76×10^7 cfu·mL^-1 by bioaugmentation,five days later,the degradation rate of naphthalene,phenanthrene,fluorene,pyrene,fluoranthene reached（59.27±8.54）%,（44.35±10.79）%,（66.64±2.35）%,（26.40±6.36）% and（53.86±4.07）%,respectively.

关 键 词：多环芳烃 微生物降解 假单胞菌属 微生物修复 

分 类 号：X172[环境科学与工程—环境科学]