菊酯类杀虫剂降解菌JZL-3(Arthrobacter sp.)的分离鉴定与降解特性研究  

Identification and degradation characteristics of a pyrethroid pesticides degrading Achromobacter sp.JZL-3

作　　者：李恋[1] 郑金伟[1] 杭宝剑[1] 王保战[1] 何健[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《安全与环境学报》2010年第6期67-71,共5页Journal of Safety and Environment

基　　金：国家自然科学基金项目(30970099);国家863项目(2006AA10Z402);江苏省自然科学基金项目(BK2008331)

摘　　要：自农药厂废水中分离到一株广谱菊酯类杀虫剂降解菌JZL-3,经过形态、生理生化试验及16S rDNA序列分析,鉴定其属于节杆菌属(Arthrobacter sp.)。该菌能降解目前市场上使用的7种主要菊酯类杀虫剂,降解速率由高到低为:氯菊酯、甲氰菊酯、氯氰菊酯、氰戊菊酯、联苯菊酯、功夫菊酯和溴氰菊酯。对氯氰菊酯的最适降解温度为30℃,最适pn值为7.0,初始接种量在一定范围内(1%～5%)与降解率呈正相关。菌株JZL-3对氯菊酯和氯氰菊酯顺反异构体的降解没有显著的差异,不同于已有的关于菊酯类杀虫剂生物降解具有显著立体异构选择性的结论。The degradation characterizatics of pyrethroid pesticidesdegrading bacterium JZL - 3, which was isolated from activated sludge collected from wastewater-treating system of a pesticide manufacturer, was studied. The strain was identified as Arthrobacter sp. according to its physiological ＆ biochemical characteristic and the similarity analysis of its 16S rDNA sequence and was able to degrade about 90% cypermethrin （100 rag/L） in 72 hours. Strain JZL - 3 was capable of degrading a wide range of pyrethroid pesticides, including fenpropathrin, permethrin, eypermethrin, fenvalerate, dehamelthrin, cyhalothrin and bifenthrin. Fenpropathrin was the most preferred substrate, permethrin, cypermethrin and fenvalerate were degraded slower than fenpropathrin, while much faster than bifenthrin, cyhalothrin and dehamethrin. Bifenthrin was the most persistent. These results indicated that substitutions of methyl with α-cyano group, dichlorocthylene or chloridbcn had little influence on the degradation efficiencies, while the substitutions of chloroyl with bromovinyl, fluoro, on the chrysanthemic acid and the replacement of the 3-phenoxybenzyl with a biphenyl significantly reduced the degradation efficicncies. The optimal temperature and pH for the degradation was 30 ℃ and 7.0, respectively. The degradation efficiency was related positively to initial inoculum size （ 1% - 5 % ） . When the initial inoculum size was more than 7 % , the degradation efficiency was not been effected. The strain could degrade cypermethrin rapidly when initial concentration was 20 - 50 mg/L, while the degradation rate was significantly reduced when initial concentration was more than 100 mg/L. No cis-trans isomer selectivity was observed to the biodegradation of permethrin and cypermethrin; this feature is different from reported results. The results of degradation characterizatics to pyrcthroid pesticides indicated that strain JZL- 3 has potential use for bioremediation of pyrethroid-contaminated soils and waters.

关 键 词：微生物学 菊酯类杀虫剂 生物降解 节杆菌属 立体异构选择性 

分 类 号：Q939.9[生物学—微生物学]