视网膜色素上皮移植细胞超微结构的观察  

The study of ultrastructure of retinal pigment epithelium of transplantation

作　　者：李光辉[1,2] 吴乐正[1,2] 高汝龙[1,2] 吴德正 

机构地区：[1]第一军医大学附属南方医院眼科 [2]中山医科大学中山眼科中心

出　　处：《中华眼科杂志》1999年第1期50-51,I002,共3页Chinese Journal of Ophthalmology

摘　　要：目的观察视网膜色素上皮移植细胞的超微结构，为临床应用提供实验基础。方法取有色兔眼球后段，去除视网膜的神经上皮层，获得视网膜色素上皮细胞作为移植的供体细胞。以１０只无色兔为移植的受体，内路法将供体色素上皮细胞移植到视网膜下腔，电镜观察移植细胞的超微结构。结果移植的１０只眼，细胞成功移植到视网膜下腔，可见移植细胞贴附于受体的Ｂｒｕｃｈ膜并形成基底内褶，细胞间紧密连接，细胞内可见吞噬体。结论内路法是一种确实可行的视网膜色素上皮移植方法，移植后的细胞可存活进行新陈代谢，形成一系列的正常超微结构。Objective To investigate the ultrastructure of retinal pigment epithelium (RPE) for the base of clinical application. Methods Pigmented rabbit RPE was transplanted to the subretinal space of 10 unpigmented rabbits by internal route. The ultrastructure of transplanted RPE cells were observed by electron microscopy. Results The transplantation of RPE was successful. It was shown that the transplanted cells had polarity and attached to Bruch′s membrane to form basal infoldings, and the relationships between transplanted cells and the host photoreceptors were also seen. Conclusions The internal approach is a practical and useful method for transplantation of RPE. The transplanted RPE can vitalize, perform metabolism and form some normal ultrastructure.

关 键 词：移植 视网膜色素上皮 超微结构 

分 类 号：R774.1[医药卫生—眼科]