血链球菌部分基因克隆及与nif基因同源序列初探  

Partial Gene Clone and nif Gene Homologous Sequence Analysis of Streptococcus sanguis

作　　者：潘亚萍[1] 王红杨[1] 金春元[1] 孙开来[1] 

机构地区：[1]中国医科大学

出　　处：《华西口腔医学杂志》1999年第1期11-13,I002,共4页West China Journal of Stomatology

基　　金：美国中华医学会CMB基金

摘　　要：目的：分析血链球菌染色体ＤＮＡ片段序列，探讨血链球菌遗传生物学特性。方法：采用ＴＡ克隆技术将此片段转化克隆并进行碱基序列测定，通过ＧｅｎＢａｎｋ进行同源分析。结果：血链球菌ＡＴＣＣ１０５５６ＤＮＡ片段含有与固氮菌高度同源的ｎｉｆＳ、ｎｉｆＵ基因，同源分值为１１４点（ｂｉｔｓ）。结论：国内外首次报道血链球菌ＤＮＡ片段含有ｎｉｆＳ、ｎｉｆＵ部分基因。Objective: To analyze the sequence of Streptococcus sanguis chromosome which contains one DNA fragment of 800 base pairs (bp) and discuss Streptococcus sanguis biological features of heredity. Methods: Streptococcus DNA of 800 bp genetic fragment was cloned and analyzed by using eukaryotic expression vector. Results:By Genebank's database, it showed that the 800 bp genetic sequence was highly homologous with other bacterial nifS and nifU gene, and the highest homologous score was 114. Conclusion: this nif gene of ATCC 10556 strain may correlate with nutrient metabolism and peroxide hydrogen release of Streptococcus sanguis.

关 键 词：血链球菌 基因克隆 nif基因 牙周炎 

分 类 号：R781.420.2[医药卫生—口腔医学]