抗变形链球菌SAⅠ/Ⅱ单抗重链可变区基因克隆及序列分析  

Cloning and Sequencing of Variable Region Gene of Heavy Chain of Monoclonal Antibody Against SA Ⅰ/Ⅱ of Streptococcus mutans

作　　者：文立亚 闫岩[1,2] 尹文 吴兴安[1,2] 姜绍淳 马文煜[1,2] 

机构地区：[1]北京市海淀区黑山沪总后309医院口腔科 [2]西安市第四军医大学微生物学教研室

出　　处：《华西口腔医学杂志》1999年第1期14-16,I002,共4页West China Journal of Stomatology

摘　　要：目的：从抗变形链球菌ＳＡⅠ／Ⅱ单抗杂交瘤细胞系２Ｂ１２Ｆ６中扩增克隆重链可变区基因。方法：采用ＰＣＲ技术和基因工程技术，扩增杂交瘤细胞系２Ｂ１２Ｆ６的重链可变区基因并克隆入载体ｐＵＣ１８，Ｓａｎｇｅｒ双脱氧链末端终止法测定核苷酸序列。结果：重链可变区基因全长３６０ｂｐ，编码１１８个氨基酸，其框架区与发表的小鼠重链可变区基因序列有７０％的同源性，符合鼠重链可变区基因的结构特征。结论：抗变形链球菌ＳＡⅠ／Ⅱ单抗杂交瘤细胞系２Ｂ１２Ｆ６重链可变区基因的获得是构建抗变形链球菌ＳＡⅠ／Ⅱ基因工程抗体的基础。Objective:To clone and sequence a immunoglobulin variable region of heavy chain(VH) from a mouse hybridoma 2B 12 F 6, which produce monoclonal antibody against SA Ⅰ/Ⅱ of Streptococcus mutans.Methods: The immunoglobulin variable region gene of heavy chain of 2B 12 F 6 was amplified and cloned into pUC18 by using PCR technique and gene engineering technique, and then the gene sequence was analyzed by Sanger's method.Results: The VH gene segment was 360 base pairs in length and coded 118 amino acids, and the homology of framework of VH gene and mouse VH gene published was 70%, which accorded with the feature of mouse VH gene. Conclusion: The VH gene gained from 2B 12 F 6 could provide the possibility of construction of gene engineering antibody against SA Ⅰ/Ⅱ of Streptococcus mutans.

关 键 词：变形链球菌 单克隆抗体 可变区基因 龋齿 

分 类 号：R781.101[医药卫生—口腔医学]