表达白细胞介素-1β转换酶逆转录病毒载体的构建及其对卵巢癌细胞生物学特性的影响  

The Influences of Interleukin 1β Converting Enzyme Expression on the Biologic Characteristics of Ovarian Cancer Cells

作　　者：李进[1] 孔宪涛[1] 钱其军 贾林 赵亚南 沙金燕 

机构地区：[1]笫二军医大学长海医院妇产科

出　　处：《中华妇产科杂志》1999年第2期113-115,I005,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨白细胞介素１β转换酶的表达，对卵巢癌细胞生物学特性的影响。方法采用分子克隆方法，将人白细胞介素１β转换酶（ＩＣＥ）基因全序列正向插入哺乳动物表达载体（ｐＬＸＳＮ）的ＥｃｏＲⅠ位点，构建ｐＬＸＳＮＩＣＥ重组体，用电穿孔方法将其导入逆转录病毒载体包装细胞系ＰＡ３１７细胞内，收集病毒上清液，再转染人卵巢癌细胞ＡＯ和３ＡＯ。结果所构建的人ＩＣＥ基因逆转录病毒载体能在肿瘤细胞内稳定表达。基因转染后，表达ＩＣＥ基因的细胞的克隆数明显较空载体减少，细胞生长速度明显减慢，并出现自发的凋亡现象；在裸鼠体内的致瘤性明显减弱。结论ＩＣＥ基因的表达，能抑制肿瘤细胞的生长，促进肿瘤细胞的凋亡，并影响肿瘤细胞在裸鼠体内的致瘤能力。Objective To investigate the effects of interleukin 1 β converting enzyme (ICE) gene on the biologic characteristics of ovarian cancer cells. Methods A 1.3 Kilobase human ICE cDNA with 5' and 3' untranslated sequences was cloned into the EcoR I site of pLXSN retrovirus vector in sense orientation. After that, the recombinant plasmid pLXSN ICE was transferred to virus packing cell PA317 by electroporation method. And then the retrovirus containing human ICE cDNA generated by these PA317 cells were used to transfect human ovarian cancer cell lines, AO and 3AO. Results After being transfected with these retrovirus, the number of the clones obtained after G418 resistance selection is far less than that of controlled groups with plasmid pLXSN. The growth rate of these transfected cells was significantly suppressed in comparison with the parental cell line. Apoptosis was also found in these cells. After the transfection cancer cells were inoculated subcutaneously in nude mice, the tumor growth was significantly inhibited. Conclusion It suggests that human ICE gene can suppress the phenotype and tumorigenicity in nude mice of ovarian cancer cell lines AO and 3AO.

关 键 词：半胱氨酸蛋白酶 卵巢肿瘤 基因表达 ICE 

分 类 号：R737.310.2[医药卫生—肿瘤]