3-甲基胆蒽诱发大鼠颌下腺肿瘤的实验研究  

Rat Submandibular Gland Tumor Induced by 3 methylcholanthrene

作　　者：曹选平[1,2] 刘学军[1,2] 何巍[1,2] 刘学杰[1,2] 郝志芳 赵庆夏 

机构地区：[1]河南医科大学口腔系 [2]河南省肿瘤病理学重点实验室

出　　处：《华西口腔医学杂志》1999年第2期128-130,I008,共4页West China Journal of Stomatology

摘　　要：目的：研究涎腺肿瘤的发生和发展规律，建立涎腺肿瘤动物模型。方法：采用化学致癌物３－甲基胆蒽对４０只ＳＤ大鼠颌下腺腺体内进行直接注射的方法，观察诱发产生颌下腺肿瘤情况。结果：共诱发肿瘤３２例，其中鳞癌４例，平滑肌肉瘤２８例。并对所诱发的两种肿瘤的组织学、免疫组织化学和超微结构进行了观察，探讨了３－甲基胆蒽对大鼠颌下腺的致癌作用及所诱发肿瘤的组织起源。结论：３－甲基胆蒽诱发大鼠颌下腺肿瘤能成功地建立颌下腺肿瘤的动物模型。Objective:To Study the genesis and the development of salivary gland tumor (SGT).Methods:SGT animal models were established by injection of 3 methylcholanthrene (3 MCA) oil solution into 40 submandibular glands of Sprague Dawley (SD) rats, then histopathology and ultrastructure of induced tumors were observed under microscope, and immunohistochemical detection of keratin and actin was carried out.Results: 32 models of submandibular gland tumors were induced successfully, with 4 rats of squamous cell carcinoma and 28 leiomyosarcoma, and the carcinogenesis of 3 MCA and tissue genesis of induced tumors were discussed.Conclusion: Submandibular gland tumor can be induced by 3 MCA, and sarcoma was of high incidence.

关 键 词：3-甲基胆蒽 大鼠 颌下腺肿瘤 化学诱癌 

分 类 号：R739.87[医药卫生—肿瘤]