PRL-3和RhoC在A549细胞中的表达及意义  

Expressions and Significances of PRL-3 and RhoC in A549 Cell

作　　者：张平[1] 张志培[1] 李香敏[2] 雷杰[1] 苏凯[1] 李小飞[1] 王小平[1] 

机构地区：[1]第四军医大学唐都医院胸外科,西安710038 [2]青海大学附属医院肾内科,西宁810001

出　　处：《中国肺癌杂志》2010年第12期1160-1164,共5页Chinese Journal of Lung Cancer

摘　　要：背景与目的在前期研究中,我们发现在非小细胞肺癌中PRL-3和RhoC的表达具有相关性,提示两者可能存在相互作用。本研究通过检测两者在A549细胞迁移中的作用以及是否存在相互作用,为进一步研究它们在肿瘤发生发展中的作用机理提供实验依据。方法应用PRL-3抗体、RhoC抗体分别阻断A549细胞中PRL-3和RhoC的功能,采用细胞划痕实验检测两者对其迁移能力的影响;应用RT-PCR检测PRL-3和RhoC表达的变化。结果 PRL-3和RhoC功能被阻断后,A549细胞迁移能力明显降低;阻断PRL-3的A549细胞中RhoC表达降低,然而阻断RhoC的A549细胞中PRL-3表达无明显改变。结论 PRL-3和RhoC可能具有促进A549细胞远处转移的功能;PRL-3可能具有上调RhoC表达的功能;PRL-3促进癌细胞远处转移的功能可能是通过RhoC及其下游因子实现的,而RhoC对PRL-3的表达可能无反馈性调节作用。Background and objective The expression of phosphatase of regenerating liver-3（PRL-3） is correlated with Ras homologue C（RhoC） in non-small cell lung cancer（NSCLC） ,suggesting that they have interactions. The aim of this study is to investigate the functions of PRL-3 and RhoC in the migration of A549 cell and the potential mechanism of PRL-3 and RhoC in carcinogenesis and cancer development. Methods PRL-3Ab and RhoCAb were used to block the functions of PRL-3 and RhoC respectively. Wound healing assay was applied to detect the migration of A549 cell and the expression levels of PRL-3 and RhoC were detected by RT-PCR. Results The migration of A549 cell decreased after blockage of PRL-3 and RhoC. The expression of RhoC decreased when PRL-3 was blocked without any changes on the expression of PRL-3. Conclusion PRL-3,RhoC could increase cell migration in A549 cells.

关 键 词：PRL-3 细胞划痕实验 细胞培养 

分 类 号：R734.2[医药卫生—肿瘤]