IFN-γ对人胃腺癌Survivin分子通路的调控作用  

Regulatory effect of IFN-γon the survivin signaling pathway in gastric adenocarcinoma

作　　者：邓昊[1] 黄萱[2] 高友晶[2] 镇鸿燕[1] 刘丽江[3] 

机构地区：[1]江汉大学病理诊断所,湖北省武汉市430056 [2]江汉大学医学院病理学与病理生理学教研室,湖北省武汉市430056 [3]江汉大学肿瘤研究所,湖北省武汉市430056

出　　处：《世界华人消化杂志》2010年第30期3249-3253,共5页World Chinese Journal of Digestology

基　　金：武汉市青年科技晨光计划基金资助项目;No.20045006071-7;湖北省自然科学基金资助项目;No.2006AB191~~

摘　　要：目的:探讨胃癌中可能存在的Survivin通路以及IFN-γ对该通路可能存在的调控.方法:人胃癌组织经免疫组织化学染色行相关性分析,SGC7901细胞经IFN-γ及Survivin反义寡核苷酸处理,应用RT-PCR和免疫细胞化学及图像分析的方法检测mRNA及蛋白水平的改变,应用Hoechst33258染色观察细胞凋亡.组织学指标相关性采用Spearman等级相关分析,平均光密度值采用t检验分析.结果:人胃癌组织中Survivin与caspase-7正相关(P=0.03);p21waf与caspase-7正相关(P=0.02);胃癌S G C7901细胞,经不同浓度S u r-vivin反义寡核苷酸处理后,Survivin蛋白表达明显下降(P<0.05),caspase-7和p21waf无明显变化.经IFN-γ处理后,Survivin mRNA表达明显下降(P<0.05),p21waf和caspase-7mRNA表达明显上升(P<0.05).经IFN-γ和Survivin反义寡核苷酸处理后细胞无明显凋亡.结论:胃癌中Survivin表达与caspase-7及p21waf相关,但不能通过调节caspase-7及p21waf的表达发挥其抑制凋亡作用;IFN-γ可使Survivin表达下调,同时上调其下游促凋亡分子caspase-7以及p21waf的表达,以发挥其生物学作用.AIM：To characterize the role of survivin in the pathogenesis of gastric adenocarcinoma and to investigate the regulatory effect of interferon-γ（IFN-γ） on the survivin signaling pathway in gastric adenocarcinoma. METHODS：Protein expression was examined by immunohistochemistry.After IFN-γand antisense oligonucleotide against survivin were used to treat SGC7901 cells,mRNA and protein expression was tested by RT-PCR and immunocytochemistry,respectively,and apoptosis was determined by Hoechst 33258 staining.Statisti-cal analysis was performed using the Spearman’ s rank correlation test and student＇s t-test. RESULTS：There was a positive correlation between caspase-7 and survivin expression（P =0.03） as well as between caspase-7 and p21 waf（P=0.02） expression in gastric adenocarcinoma. Treatment with survivin antisense oligonucleotide down-regulated the protein expression of survivin（P〈0.05） but had no significant impact on caspase-7 and p21 waf protein expression（both P〉0.05） .IFN-γtreatment down-regulated the mRNA expression of survivin（P〈0.05） but upregulated the mRNA expression of caspase-7 and p21 waf（both P〈0.05） .Treatment with either IFN-γor survivin antisense oligonucleotide had no significant impact on cell apoptosis. CONCLUSION：Although the expression of survivin is correlated with that of caspase-7 and p21 waf,survivin can not regulate apoptosis in gastric adenocarcinoma by regulating caspase-7 and p21 waf expression.IFN-γcan regulate apoptosis in gastric adenocarcinoma by down-regulating survivin expression and up-regulating caspase-7 and p21 waf expression.

关 键 词：生存素 CASPASE-7 p21waf 干扰素-Γ 胃腺癌 

分 类 号：R735.2[医药卫生—肿瘤]