青鳉DMY基因的克隆及其在性别鉴定中的应用  

Gene Cloning and Application of DMY Gene for Sex Identification in Oryzias latipes

作　　者：杨明[1] 王昆鹏[1] 张玉颖[1] 徐刚[1] 吴明红[1] 

机构地区：[1]上海大学环境与化学工程学院,上海200444

出　　处：《上海大学学报（自然科学版）》2010年第6期562-566,共5页Journal of Shanghai University:Natural Science Edition

基　　金：国家自然科学基金资助项目(40830744;40973073;40973072);上海高校选拔培养优秀青年教师科研专项基金资助项目(SHU08021);上海大学创新基金资助项目(A10011109006)

摘　　要：提取雄性青鳉成鱼基因组DNA为模板,利用特异性引物,聚合酶链式反应(polymerase chain reaction,PCR)扩增获得1 908 bp的特异性条带.经测序证明为DMY基因片段,其核苷酸序列由3个外显子和2个内含子组成,可连续编码150个氨基酸.建立准确而有效的鉴定任意发育阶段青鳉个体雌雄性别基因型的方法,即利用管家基因——肌动蛋白基因PCR扩增条带的有无验证DNA样本提取和PCR扩增的有效性,同时利用雄性DMY基因的PCR扩增条带的有无鉴定青鳉个体的雌雄性别.对随机选取的成鱼和新生鱼卵样本,应用该方法可成功地鉴定出青鳉个体的雌雄性别.Genomic DNA was extracted from a male Japanese medaka （Oryzias latipes ） and a DNA fragment of 1 908 bp in length was cloned using specific primers by polymerase chain reaction （PCR） amplification. DMY gene was then identified after sequencing, which consisted of three exons and two introns and encoded a peptide of 150 amino acids. In our study, an accurate and effective method was established for sex identification （ gene type） of any development stage of medaka, which is amplifying household 13-actin gene to testify the DNA template and PCR amplification, and then amplifying specific DMY gene to distinguish the gender of medaka. The method has been successfully applied to sex identification in randomly selected mature medaka and new born fish eggs.

关 键 词：日本青鳉 性别决定基因 DMY基因 

分 类 号：X17[环境科学与工程—环境科学]