纤维素酸解副产物对Clostridium acetobutylicum CICC8012发酵的影响  

Effects of Byproducts from Acid Hydrolysis of Lignocellulose on Butanol Fermentation by Clostridium acetobutylicum CICC8012

作　　者：孙彦平[1,2] 靳艳玲[1] 郜晓峰[1] 李新波[1,2] 肖瑶[1,2] 赵海[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院研究生院,北京100049

出　　处：《应用与环境生物学报》2010年第6期845-850,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技支撑计划项目(No.2011BAD22B03)资助~~

摘　　要：为确定纤维素酸解副产物对C.acetobutylicum CICC8012发酵生产纤维燃料丁醇的影响,选择乙酸根、甲酸、葡萄糖醛酸、氯化钠、硫酸钠、糠醛、羟甲基糠醛(HMF)、香豆酸、阿魏酸、丁香醛、香草醛等纤维素酸解副产物,研究其对菌种发酵的影响.结果表明:甲酸和酚类物质的抑制作用强烈,0.5 g/L的甲酸、阿魏酸或香豆酸存在时对丁醇的抑制率分别为88.1%、22.1%、82.4%,丁香醛(≥0.5 g/L)、香草醛(≥2 g/L)可使发酵的延滞期变长,但抑制作用小于阿魏酸和香豆酸;1 g/L的糠醛、HMF、葡萄糖醛酸存在时对丁醇的抑制率分别为9.8%、4.6%、7.3%,10 g/L乙酸钠存在时对丁醇的抑制率为4.8%,这4种物质对丁醇发酵的抑制作用不大;硫酸钠、氯化钠质量浓度大于5 g/L时对发酵产生抑制作用,浓度达到20 g/L时菌体几乎不能生长.当各抑制物混合时,若抑制物的含量超过临界点,各抑制物间存在相互促进的作用;小麦秸秆水解液的发酵结果表明,水解液成分复杂,抑制作用更为强烈.Several byproducts of acid hydrolysis,including sodium acetate,formic acid,glucuronic acid,furfural,5-hydroxymethylfurfural,sodium chloride,sodium sulfate,ferulic acid,coumaric acid,syringaldehyde and vanillin,were added into the medium at different concentrations to investigate their effects on butanol fermentation by Clostridium acetobutylicum CICC8012.The fermentation of butanol by the strain was strongly inhibited by formic acid and phenolic compounds.The butanol inhibition rate was 88.1%,22.1% and 82.4% respectively when o.5 g/L formic acid,ferulic acid or coumaric acid existed in the medium.Syringaldehyde（≥0.5 g/L） and vanillin（≥2 g/L） caused a much longer lag phase,but their negative effects on butanol fermentation were weaker than those of ferulic acid and coumaric acid.The butanol inhibition rate was 9.8%,4.6% and 7.3% respectively when 1 g/L furfural,5-hydroxiymethylfurfural or glucuronic acid existed in the medium.The butanol inhibition rate was 4.8% when 10 g/L sodium acetate existed in the medium.The inhibiting effects by furfural,5-hydroxiymethylfurfural,glucuronic acid and sodium acetate were relatively weaker.It began to show inhibiting effects when the concentrations of sodium sulfate and sodium chloride exceeded 5 g/L.When their concentrations reached 20 g/L,the fermentation was almost inhibited.When the inhibitors were mixed together and their concentrations were above the critical point,the inhibiting effect was enhanced.The result of fermentation from wheat straw hydrolysate indicated that the composition of the hydrolysate was more complicated and the inhibiting effect was stronger.

关 键 词：纤维素 酸水解 抑制物 CLOSTRIDIUM ACETOBUTYLICUM CICC8012 丁醇发酵 

分 类 号：TK6[动力工程及工程热物理—生物能]