长期口服卡托普利对小鼠脑组织自由基及相关酶的影响  

Effect of Long-term Oral Dose of Captopril on Free Radicals and Related Enzymes of Mice Cerebral Tissue

作　　者：谢湘林[1] 马春颖[1] 周鸣[2] 叶秋芳[1] 赵莉[1] 刘宏雁[1] 

机构地区：[1]吉林大学药学院药理教研室,长春市130021 [2]长春中医药大学第一附属医院新药中心,长春市130021

出　　处：《中国药房》2011年第1期27-28,共2页China Pharmacy

摘　　要：目的:探讨长期口服卡托普利对小鼠脑组织自由基及相关酶的影响,为其临床应用提供实验依据。方法:取30只小鼠,随机均分为正常对照组(水)和卡托普利组(卡托普利,11.08～14.92mg·kg-1·d-1),按饮水给药方式连续给药14周,测定脑组织中自由基与相关酶(丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、钠-钾泵三磷酸腺苷酶(Na+-K+-ATP酶)、钙-镁泵三磷酸腺苷酶(Ca2+-Mg2+-ATP酶))水平。结果:与正常对照组比较,卡托普利组小鼠脑组织中MDA水平降低(P<0.05);SOD、GSH-Px水平均无明显变化;Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶活性有降低的趋势,但无统计学意义。结论:长期口服卡托普利可减少脑组织自由基含量,增强机体抗氧化能力,但脑组织神经细胞膜功能有受损的可能。OBJECTIVE：To explore the effect of long-term oral dose of Captopril on free radicals and related enzymes of mice cerebral tissue in order to give experimental basis for clinical use of captopril.METHODS：30 normal adult mice were divided into control group（water）and captopril group（captopril 11.08～14.92 mg·kg -1 ·d -1 ）.Both groups were given medicine for 14 weeks. Free radicals and the activity of related enzymes（MDA,SOD,GSH-Px,Na＋-K＋-ATP enzyme,Ca2＋-Mg2＋-ATP enzyme）in cerebral tissue were detected.RESULTS：Compared with control group,in captopril group the content of MDA decreased（P0.05）,the activity of SOD,GSH-Px hadn＇t changed obviously.The activity of Ca2＋-Mg2＋-ATP enzyme and Na＋-K＋-ATP enzyme declined,but there was no statistical significance.CONCLUSION：Oral does of captopril for a long time can reduce free radicals and strengthen the antioxidant function of cerebral tissue,but it may damage the neuron membrane function of cerebral tissue.

关 键 词：卡托普利 自由基 小鼠 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 钠-钾泵三磷酸腺苷酶 钙-镁泵三磷酸腺苷酶 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R965[医药卫生—临床医学]