左旋丁基苯酞对乳鼠脑胶质细胞氧糖剥夺/复氧后脑胶质细胞数量及IL-1β蛋白表达的影响  

L-3-n-butylphthalide reduces the quantity of the brain glial cells and inhibits the expression of IL-1β protein in mouse brain glial cells following oxygen-glucose deprivation/reoxygenation

作　　者：邓杉杉 罗勇[2] 

机构地区：[1]重庆市长安医院神经内科,400023 [2]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,400016

出　　处：《中国神经免疫学和神经病学杂志》2011年第1期32-36,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：重庆市科委自然科学基金资助项目(CSTC;2007BB5297)

摘　　要：目的观察左旋丁基苯酞(L-3-n-butylphthalide,l-NBP)对原代培养乳鼠脑胶质细胞氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation,OGD/R)后脑胶质细胞数量及白细胞介素-1β(IL-1β)蛋白表达的影响。方法采用DMEM培养基体外培养乳鼠脑胶质细胞,免疫细胞化学鉴定培养细胞类型,建立脑胶质细胞OGD/R损伤模型,通过测定细胞OGD/R时乳酸脱氢酶(LDH)释放量,评估脑胶质细胞OGD模型。OGD 24h/R 24 h后,采用MTT法测定脑胶质细胞数量,并观察l-NBP对OGD 24 h/R 24 h后脑胶质细胞数量的影响;采用间接酶联免疫吸附法(indirect enzyme-linked immunosorbent assay,ELISA)测定各组脑胶质细胞IL-1β蛋白表达。结果 OGD 4 h组、OGD 8 h组、OGD 12 h组、OGD 24 h组脑胶质细胞LDH释放量均比正常对照组明显增多(P<0.01);OGD不同时间组组间比较差异有统计学意义(P<0.01)。OGD 24 h/R 24 h后脑胶质细胞数量较对照组明显增加(P<0.01);l-NBP 500μmol/L组脑胶质细胞数量较OGD 24 h/R 24 h组明显减少(P<0.05)。OGD 24 h/R 24 h组IL-1β蛋白表达比对照组明显增多(P<0.05);l-NBP 100和500μmol/L组IL-1β蛋白表达较OGD 24 h/R 24 h组明显减少(P<0.05)。结论 l-NBP能减少乳鼠脑胶质细胞OGD/R后细胞数量以及IL-1β蛋白的表达。Objective To observe the effect of L-3-n-butylphthalide （1-NBP） on the expression of interleukin-1β （IL-1β） protein in primary cultured mouse brain glial cells following oxygen-glucose deprivation/ reoxygenation （OGD/R）. Methods The mouse brain glial cells were cultured with DMEM medium and identified with immunocytochemistry. The OGD/R model was established and evaluated based on the releasing amount of LDH. The quantity of brain glial cells and the expression of IL-1β protein in 1-NBP groups were analyzed by MTT and ELISA after OGD 24 h/R 24 h. Results Compared with the normal control group, each of the four groups, including OGD 4 h group, OGD 8 h group, OGD 12 h group and OGD 24 h group, had significantly higher expression of LDH （P〈0.01）; and the significant difference was also present among the four OGD groups （P〈0.01）. The quantity of brain glial cells in OGD 24 h/R 24 h group was increased in comparison with normal control group （P〈0. 01）. Compared with OGD 24 h/R Z4 h group, the quantity of brain glial cells in 1-NBP 500μmol/L group decreased apparently （P〈0.05）. The expression of IL-1β protein in OGD 24 h/R 24 h group was significantly higher than that in normal control group （P〈0. 05）. It was obvious that the expression of IL-1β protein in 1-NBP 100 μmol/L group and I-NBP 500 μmol/L group was lower than that in OGD 24 h/R 24 h group （P〈0. 05）. Conclusions I-NBP can reduce the quantity of brain glial cells and the expression of IL-1β protein of brain glial cells following OGD/R.

关 键 词：左旋丁基苯酞 胶质细胞 白细胞介素-1β 氧糖剥夺/复氧 

分 类 号：R743[医药卫生—神经病学与精神病学]