8-Br-cAMP对人视网膜母细胞瘤HXO-Rb44细胞癌基因的表达效应  

The effect of 8 Br cAMP on oncogene expression in human retinoblastoma HXO Rb44 cells

作　　者：邓新国[1] 吴景兰[1] 王翔[1] 田小莉[1] 庞广仁[1] 

机构地区：[1]河南省眼科研究所

出　　处：《眼科研究》1999年第4期244-246,共3页Chinese Ophthalmic Research

基　　金：河南省科委资金

摘　　要：目的研究８ＢｒｃＡＭＰ对培养的人视网膜母细胞瘤ＨＸＯＲｂ４４细胞癌基因表达的效应及其与该细胞生长的关系。方法ｃｆｏｓｍＲＮＡ、ＮｍｙｃｍＲＮＡ及ｐ２１ｒａｓｍＲＮＡ均以原位杂交和ＲＮＡ斑点印迹技术检测，对繁殖细胞核抗原（ＰＣＮＡ）、ｃＦｏｓ、ＮＭｙｃ和Ｐ２１ｒａｓ蛋白表达的免疫反应性（ＩＲ）则采用免疫组化及蛋白质斑点印迹技术检测。结果在人ＨＸＯＲｂ４４细胞，ｃｆｏｓｍＲＮＡ、ＮｍｙｃｍＲＮＡ及ｐ２１ｒａｓｍＲＮＡ定位于胞质，ｃＦｏｓＩＲ及ＮＭｙｃＩＲ定位于胞核，而Ｐ２１ｒａｓＩＲ则位于胞质。斑点印迹的相对扫描数值显示实验组（８ＢｒｃＡＭＰ处理）均低于相应各对照组（未经８ＢｒｃＡＭＰ处理），Ｐ＜０．０５～０．０１。结论（１）８ＢｒｃＡＭＰ可抑制人ＨＸＯＲｂ４４细胞的生长增殖；（２）８ＢｒｃＡＭＰ可下调ｃｆｏｓ、Ｎｍｙｃ及ｐ２１ｒａｓ癌基因的表达；（３）８ＢｒｃＡＭＰ也可下调ｃＦｏｓ、ＮＭｙｃ及Ｐ２１ｒａｓ蛋白的表达，而且其变动各与其ｍＲＮＡ基因表达的变动相一致；（４）结果提示８ＢｒｃＡＭＰ可能从不同的途径而协同抑制ＨＸＯＲｂ４４细胞的生长增殖。Objective To study the effect of 8 Br cAMP on oncogene expression and cell growth in human cultured retinoblastoma cells. Methods The c fos,N myc and p21ras mRNAs were detected by in situ hybridization and RNA dot blot techniques.The immunoreactivity (IR) of proliferative cell nuclear antigen (PCNA),c Fos,N Myc and P 21ras were detected by immunohistochemistry and protein dot blot techniques. Results In the HXO Rb44 cells the signals for c fos mRNA,N myc mRNA,and p21ras mRNA were localized in the cytoplasm.The c Fos IR,N Myc IR were localized in the nuclei,while P 21ras IR localized in the cytoplasm.The relative scanning value of all dot blots in the experimental groups (treated with 8 Br cAMP) was lower than that in respective control groups(treated with no 8 Br cAMP)( P <0.05～0.01). Conclusions 8 Br cAMP down regulates the gene expression of c fos,N myc and p21ras to inhibit the HXO Rb44 cell growth and proliferation.The results suggest that the effect of 8 Br cAMP on inhibition of HXO Rb44 cell proliferation may occur through synergic action of various oncogenes.

关 键 词：视网膜母细胞 HXO-RB44 8-BR-CAMP 癌基因 

分 类 号：R739.702[医药卫生—肿瘤]