骆驼蓬总碱及其脂质体对兔晶状体上皮细胞的抑制作用  

Effect of liposome encapsulated total alkaloid of harmaline on lens epithelial cells in rabbit eyes

作　　者：吴明星[1] 利华明[1] 李绍珍[1] 潘苏华[1] 梁舜薇[1] 郑湖玲[1] 

机构地区：[1]中山医科大学中山眼科中心

出　　处：《眼科研究》1999年第4期275-278,共4页Chinese Ophthalmic Research

基　　金：广东省自然科学基金资助

摘　　要：目的探讨骆驼蓬总碱（ＴＡＨ）及其脂质体（ＬＴＡＨ）对兔后囊混浊（ＰＣＯ）形成的影响。方法用改良冻融法制备ＬＴＡＨ。１０只健康纯种新西兰白兔分别每眼行晶状体囊外摘出术并分别注入０１ｍｌ浓度为０２ｍｇ／ｍｌＴＡＨ及ＬＴＡＨ。观察术后后囊、手术前后眼压、角膜内皮细胞、视网膜电图及组织病理学变化。结果注药术后４周实验眼与对照眼角膜内皮细胞密度及形态、视网膜电图的ｂ波振幅及潜时无明显差别。组织病理学检查显示对照组眼明显残留晶状体上皮细胞（ＬＥＣ）增生并有Ｓｏｅｍｍｅｒｉｎｇ环形成，而用药眼无明显的残留ＬＥＣ增殖且细胞空泡变性。结论ＴＡＨ及ＬＴＡＨ能抑制兔眼残留ＬＥＣ增殖，有可能成为一种预防后发性白内障的药物。Objective To investigate the effect of liposome encapsulated total alkaloid of harmaline( LTAH) on lens epithelial cells(LEC) and preventing the formation of posterior capsule opacification(PCO) Methods LTAH was prepared by modified freeze thawing method. 0.1ml LTAH(0.2g/L) were injected into the capsular bag during extracapsular lens extraction (ECLE) of 10 rabbit eyes. Data on intraocular pressure, density of corneal endothelial cells,the b wave of ERG as well as the histopathological examination were collected. Results PCO formation was found both in TAH treated and control group by 4 weeks,however, it was much milder in LTAH group. There was no difference in the density of corneal endothelial cells,the amplitude and latency of b wave of ERG between the LTAH eyes, and control eyes. Histopathological study revealed that LEC proliferation occured by 4 weeks and Soemmering ring formation by 8 weeks in controls, only mild vacuolization of LEC was noted in the LTAH and TAH treated eyes. Conclusion The results suggest that LTAH or TAH could inhibit proliferation of LECs without definite toxicity, therefore it may be used as a potential agent in preventing the development of PCO.

关 键 词：骆驼蓬总碱 晶状体上皮细胞 脂质体 白内障 PCO 

分 类 号：R776.105[医药卫生—眼科]